首页> 外国专利> PRIMER SET FOR DETECTING FUSARIUM OXYSPORUM F. SP. RAPHANI CAUSING WILT DISEASE IN RADISH, AND METHOD FOR DETECTING FUSARIUM OXYSPORUM F. SP. RAPHANI BY USING SAME

PRIMER SET FOR DETECTING FUSARIUM OXYSPORUM F. SP. RAPHANI CAUSING WILT DISEASE IN RADISH, AND METHOD FOR DETECTING FUSARIUM OXYSPORUM F. SP. RAPHANI BY USING SAME

机译:用于检测镰孢镰刀菌的底漆F. SP。萝卜引起萝卜枯萎病的拉帕尼,以及检测枯萎病的方法使用相同的RAPHANI

摘要

The present invention relates to a method for detecting Fusarium oxysporum f. sp. raphani by using a novel primer set. In particular, the present invention relates to a novel primer set capable of specifically detecting Fusarium oxysporum f. sp. raphani causing Fusarium wilt in radish, a composition including the same, and a method for diagnosing plant diseases by identifying Fusarium oxysporum f. sp. raphani and by detecting Fusarium oxysporum f. sp. raphani in a radish plant infected by Fusarium wilt using the same. When a polymerase chain reaction (PCR) is performed by using the primer of the present invention, only a pathogenic Fusarium oxysporum f. sp. raphani strain can be separated from various Fusarium oxysporum strains. Accordingly, Fusarium oxysporum f. sp. raphani can be separated from other similar bacteria, and Fusarium oxysporum f. sp. raphani can be effectively detected from a radish plant sample infected by Fusarium wilt. Thus, the primer of the present invention can be useful in rapidly diagnosing Fusarium wilt.;COPYRIGHT KIPO 2016
机译:本发明涉及一种检测尖孢镰刀菌f的方法。 sp。通过使用新颖的引物组来制作萝卜。特别地,本发明涉及能够特异性地检测尖孢镰刀菌f的新型引物组。 sp。引起萝卜枯萎病的萝卜,一种包括萝卜的枯萎病组合物,以及通过鉴定尖孢镰刀菌f来诊断植物病害的方法。 sp。萝卜和通过检测镰刀菌f。 sp。在使用镰刀菌枯萎病的萝卜植物中使用萝卜。当使用本发明的引物进行聚合酶链反应(PCR)时,仅致病性尖孢镰刀菌f。 sp。可以将Raphani菌株与各种尖孢镰刀菌菌株分离。因此,尖孢镰刀菌f。 sp。 Raphani可以与其他类似细菌和尖孢镰刀菌(Fusarium oxysporum f)分离。 sp。从镰刀菌枯萎病感染的萝卜植物样品中可以有效地检测出萝卜。因此,本发明的引物可用于快速诊断枯萎病。; COPYRIGHT KIPO 2016

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