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METHOD FOR INDUCING DIFFERENTIATION OF ADIPOSE STEM CELLS INTO NEURAL STEM CELLS, NEURONS AND GAMMA-AMINOBUTYRIC ACID NEURONS, AND METHOD FOR INDUCING DIFFERENTIATION OF HUMAN STEM CELLS THAT SECRETE LARGE AMOUNTS OF GROWTH FACTORS FROM HUMAN BONE MARROW DERIVED MESENCHYMAL STEM CELLS
METHOD FOR INDUCING DIFFERENTIATION OF ADIPOSE STEM CELLS INTO NEURAL STEM CELLS, NEURONS AND GAMMA-AMINOBUTYRIC ACID NEURONS, AND METHOD FOR INDUCING DIFFERENTIATION OF HUMAN STEM CELLS THAT SECRETE LARGE AMOUNTS OF GROWTH FACTORS FROM HUMAN BONE MARROW DERIVED MESENCHYMAL STEM CELLS
The present invention relates to a method of differentiating adipocyte derived mesenchymal stem cells into neural stem cells, neurons and gamma-aminobutyric acid neurons. More specifically, the present invention has established a method which cultures adipocyte derived mesenchymal stem cells in a cell culture medium to which low molecular inhibitors SB431543, Noggin, and LDN193189 are added for 6 to 8 days (step 1: pre-induction step), then cultures the adipocyte derived mesenchymal stem cells in a medium to which B27, N2, and ascorbic acid are added for 5 days (step 2: neural induction step), further cultures the adipocyte derived mesenchymal stem cells in a medium to which bFGF and EGF are added for 5 to 7 days (step 3: proliferation step) to differentiate the same into neural stem cells, further differentiates the neural stem cells into neurons using a medium where purmorphamine and a brain-derived neurotrophic factor (BDNF) are added to the cell culture of step 3, and also differentiates the neurons into gamma-aminobutyric acid neurons using a medium where purmorphamine and BDNF and dbcAMP and BDNF are added to the cell culture of step 2. The present invention may be useful for identifying molecular mechanisms associated with the development of human neurons or GABAergic interneurons. Adult mesenchymal stem cell-derived neurons or induced GABAergic intervening interneurons having the properties of a particular disease in vitro using the differentiation method of the present invention can be effectively useful for identifying a neurological disease and for screening a drug.
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