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AFFINITY PLATE FOR HAPTOGLOBIN PHENOTYPE DETERMINATION, KIT COMPRISING IT, AND METHOD OF HAPTOGLOBIN PHENOTYPE DETERMINATION BY MEANS OF AFFINITY PLATES IN COMBINATION WITH DESORPTION IONIZATION MASS SPECTROMETRY TECHNIQUES
AFFINITY PLATE FOR HAPTOGLOBIN PHENOTYPE DETERMINATION, KIT COMPRISING IT, AND METHOD OF HAPTOGLOBIN PHENOTYPE DETERMINATION BY MEANS OF AFFINITY PLATES IN COMBINATION WITH DESORPTION IONIZATION MASS SPECTROMETRY TECHNIQUES
The invention relates to the affinity plate for haptoglobin phenotype determination, the kit comprising it, and the method of haptoglobin phenotype determination by detection of its a subunits by means of mass spectrometry desorption ionization techniques after preceding preconcentration of haptoglobin on the surface of the affinity plate with immobilized anti-haptoglobin antibody that was deposited onto this surface from gas phase after preceding electrospray ionization and heat desolvation. Then the signals of mass spectra corresponding to α1 and/or α2 subunits can be found and thus determined the haptoglobin phenotype, whereas only al subunit exists in phenotype 1-1, both the subunits al and α2 exist in phenotype 2-1, and only α2 subunit exists in phenotype 2-2.
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