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BIOTRANSFORMATION METHOD FOR TRANSFORMING PHENOLIC COMPOUNDS FROM SOY EXTRACT INTO EQUOL AND BIOACTIVE ISOFLAVONES BY FERMENTATION AND/OR ENZYMATIC APPLICATION, THUS OBTAINED COMPOSITION AND USE
BIOTRANSFORMATION METHOD FOR TRANSFORMING PHENOLIC COMPOUNDS FROM SOY EXTRACT INTO EQUOL AND BIOACTIVE ISOFLAVONES BY FERMENTATION AND/OR ENZYMATIC APPLICATION, THUS OBTAINED COMPOSITION AND USE
The present invention relates to a biotransformation method for transforming phenolic compounds from soy milk into bioactive isoflavones and equol, with a high yield and production volume, for use in the biotechnological field, mainly in the food industry. The invention allows the glycosylated forms of isoflavones (daidzin and genistin) to be biotransformed into the respective aglycone forms (daldzein and genistein) by fermentation of soy milk with initiator cultures and probiotic lactic bacteria, and/or by treatment of the extract with the enzyme tanase from Paecilomyces variotti. In one of the embodiments, the present invention relates to an in vitro method for producing bioactive equol in the absence of intestinal bacteria, using only enzymatic biotransformation.
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