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Methods for genetic study and analysis of populations

机译:群体遗传研究和分析方法

摘要

1. A method for building a database of profiles variety of labels (PDM) comprising the steps of: a) sequencing a plurality of markers rRNA of a sample, wherein said sample comprises a microbial population, and wherein each marker comprises a polymorphic rRNA gene sequence microbial rRNA and wherein the plurality of markers rRNA provides an accurate representation of the microbial population from said sample; b) determining the abundance in the sample, each of said plurality of markers rRNA; c) transducing the abundance of each marker rRNA in an electrical output signal; d) storing said electrical output signals produced in step c) in a matrix data structure and associating, in said structure, each output signal with the corresponding marker sequence rRNA whose abundance became an output signal; e) providing the abundances of one or more sample parameters that are associated with said sample; f) transducing the abundances of each parameter shows an electrical output signal; g) storing said electrical output signals produced in step f) in a matrix data structure associating in said structure and each output signal with parameter whose abundance sample became an output signal; h) designed as PDM signals electrical output produced in corresponding to the plurality of abundances markers rRNA and the electrical output signals produced in corresponding to the one or more abundances sample parameters step f) step c); i) repeating steps ah to a plurality of different samples, and design, as PDM data base, the plurality of PDM stored in said data structure.
机译:1.一种用于建立标记物谱(PDM)概况数据库的方法,其包括以下步骤:a)对样品的多个标记物rRNA进行测序,其中所述样品包含微生物种群,并且其中每个标记物包括多态性rRNA基因。对微生物rRNA进行测序,其中多个标记物rRNA提供了所述样品中微生物种群的准确表示; b)确定样品中所述多个标记rRNA中的每一个的丰度; c)在电输出信号中转导每个标记rRNA的丰度; d)将步骤c)中产生的所述电输出信号存储在矩阵数据结构中,并且在所述结构中将每个输出信号与相应的标记序列rRNA相关联,所述标记序列rRNA的丰度变为输出信号; e)提供与所述样本相关联的一个或多个样本参数的丰度; f)转换每个参数的丰度以显示电输出信号; g)将在步骤f)中产生的所述电输出信号存储在矩阵数据结构中,该矩阵数据结构与所述结构相关联,并且每个输出信号具有参数,所述参数的丰度样本变为输出信号; h)将步骤f),步骤c)中对应于多个丰度标记rRNA产生的电输出和对应于一个或多个丰度样品参数产生的电输出信号设计为PDM信号; i)对多个不同的样本重复步骤ah,并且将存储在所述数据结构中的多个PDM设计为PDM数据库。

著录项

  • 公开/公告号ES2632538T3

    专利类型

  • 公开/公告日2017-09-14

    原文格式PDF

  • 申请/专利权人 TAXON BIOSCIENCES INC.;

    申请/专利号ES20100012384T

  • 发明设计人 ASHBY MATTHEW;

    申请日2001-04-10

  • 分类号C12Q1/68;C07H21/02;C07H21/04;C12P19/34;G01N33/48;G01N33/50;G06F19;

  • 国家 ES

  • 入库时间 2022-08-21 13:35:11

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