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universal primers and their use for the detection and identification of species of frogs / fish
universal primers and their use for the detection and identification of species of frogs / fish
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机译:通用引物及其在青蛙/鱼类物种检测和鉴定中的用途
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摘要
Oligonucleotide pair, whereby the first oligonucleotide hybridizes selectively to SEQ ID NO: 4 and the second nucleotide hybridizes selectively to the sequence SEQ ID 5 or SEQ ID NO: 6 under conditions of stringencies sufficient for amplification of a variable region of mitochondrial gene 12S amphibians and fish by polymerase chain reaction (PCR).
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机译:寡核苷酸对,其中第一个寡核苷酸在足以扩增线粒体基因12S两栖动物和两栖动物的可变区的严格条件下与SEQ ID NO:4选择性杂交,第二个核苷酸与SEQ ID NO 5或SEQ ID NO:6选择性杂交。鱼通过聚合酶链反应(PCR)。
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