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Method for stimulating T cell responses specific antigen using dendritic cells cocultured accelerated

机译:共培养的树突状细胞刺激T细胞反应特异性抗原的方法

摘要

A method for stimulating responses of T cells specific antigen (Ag) in a sample of blood or mononuclear cells unfractionated peripheral blood (PBMC) isolated from a subject, comprising the steps of: a) culturing said blood sample or unfractionated PBMC sample in a medium that induces differentiation of dendritic cells (DC), said means colony stimulating factor granulocyte-macrophage (GM-CSF) and interleukin 4 (IL-4); b) said mature DC; in which Ag is added during steps a) and / or b).
机译:刺激从受试者分离的血液或单核外周血(PBMC)样品中的T细胞特异性抗原(Ag)的应答的方法,其包括以下步骤:a)在培养基中培养所述血液样品或未分级PBMC样品诱导树突状细胞(DC)分化的,表示集落刺激因子粒细胞巨噬细胞(GM-CSF)和白介素4(IL-4); b)说成熟的DC;其中在步骤a)和/或b)中添加了Ag。

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