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Method for stimulating T cell responses specific antigen using dendritic cells cocultured accelerated
Method for stimulating T cell responses specific antigen using dendritic cells cocultured accelerated
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机译:共培养的树突状细胞刺激T细胞反应特异性抗原的方法
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摘要
A method for stimulating responses of T cells specific antigen (Ag) in a sample of blood or mononuclear cells unfractionated peripheral blood (PBMC) isolated from a subject, comprising the steps of: a) culturing said blood sample or unfractionated PBMC sample in a medium that induces differentiation of dendritic cells (DC), said means colony stimulating factor granulocyte-macrophage (GM-CSF) and interleukin 4 (IL-4); b) said mature DC; in which Ag is added during steps a) and / or b).
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