首页> 外国专利> PLANT TRANSFORMATION VECTOR FOR SUPPRESSING MIPS GENE EXPRESSION AND METHOD FOR CULTURING LOW PHYTATE SOYBEAN

PLANT TRANSFORMATION VECTOR FOR SUPPRESSING MIPS GENE EXPRESSION AND METHOD FOR CULTURING LOW PHYTATE SOYBEAN

机译：抑制mIPS基因表达的植物转化载体和低植酸大豆的培养方法

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摘要

Plant transformation vector for suppressing MIPS gene expression and method for culturing low phytate soybean provides a plant transformation vector and method for transforming soybean plants. The vector is design to suppress or enhance gene of interest in plants. The plant transformation vector comprises a plant active promoter, a terminator, a plant selection marker and a bacterial selection marker. The present invention also provides a method of transformation for reducing phytate in soybean comprises growing a soybean plant comprising MlPS"intron hairpin expression cassette operably linked to a promoter {vicilin) that drives tissue specific expression in plants. The expression of this novel RNAi hairpin cassette for silencing MIPS gene disclosed here in confers advantageous nutritional properties i.e. reduced phytate content with increased inorganic phosphorous and mineral bioavailability, and leads to valuable and useful soybean products.

机译：用于抑制MIPS基因表达的植物转化载体和低植酸大豆的栽培方法提供了用于转化大豆植物的植物转化载体和方法。设计该载体以抑制或增强植物中的目的基因。植物转化载体包含植物活性启动子，终止子，植物选择标记和细菌选择标记。本发明还提供了一种用于减少大豆中植酸的转化方法，该方法包括生长包含与可驱动植物中的组织特异性表达的启动子（维西林）有效连接的MlPS”内含子发夹表达盒的大豆植物。这种新型RNAi发夹盒的表达使本文公开的MIPS基因沉默的方法具有有利的营养特性，即降低的肌醇六磷酸含量与增加的无机磷和矿物质的生物利用度，并产生有价值和有用的大豆产品。

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公开/公告号IN2015DE02432A

专利类型
公开/公告日2017-02-10

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申请/专利号IN2432/DEL/2015
发明设计人 MRS MANSI PUNJABI;MS VANITA PANDEY;MR ALKESH HADA;MRS VEDA KRISHNAN;MR AWADHESH KUMAR;MR ASHISH MARATHE;MRS NABNEETA BASAK;MRS MONICA JOLLY;DR ARCHANA SACHDEV;
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申请日2015-08-07
分类号C12N15/00;
国家 IN
入库时间 2022-08-21 13:38:43

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