首页> 外国专利> METHODS FOR THE SYNTHESIS OF A NUCLEIC ACID MOLECULE, TO SELECTIVELY AMPLIFY A SEQUENCE OF NUCLEIC ACID TARGETED FROM A DNA OR A MIXTURE OF NUCLEIC ACIDS a nucleic acid molecule with genetic diversity to detect a target nucleotide sequence in a sample of the nucleic acid and to allow a ring specificity of an oligonucleotide to be determined by a structure of the oligonucleotide and oligonucleotide OF DUAL SPECIFICITY

METHODS FOR THE SYNTHESIS OF A NUCLEIC ACID MOLECULE, TO SELECTIVELY AMPLIFY A SEQUENCE OF NUCLEIC ACID TARGETED FROM A DNA OR A MIXTURE OF NUCLEIC ACIDS a nucleic acid molecule with genetic diversity to detect a target nucleotide sequence in a sample of the nucleic acid and to allow a ring specificity of an oligonucleotide to be determined by a structure of the oligonucleotide and oligonucleotide OF DUAL SPECIFICITY

机译：合成核酸分子的方法，用于选择性扩增从DNA或核酸混合物中靶向的核酸序列，该核酸分子具有遗传多样性，可检测核酸样品中的目标核苷酸序列，并允许通过寡核苷酸和寡核苷酸的结构确定寡核苷酸的环特异性

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摘要

methods for synthesizing a nucleic acid molecule, to selectively amplify a target nucleic acid sequence of a DNA or a nucleic acid mixture, to amplify two or more target nucleotide sequences simultaneously, to sequence a target nucleic acid molecule for detecting a genetically diverse nucleic acid molecule, for detecting a target nucleotide sequence in a nucleic acid sample, and for allowing an annular specificity of an oligonucleotide to be determined by means of an oligonucleotide structure, and, oligonucleotide of dual specificity. The present invention relates to various processes for a template dependent extension reaction using a dual specificity oligonucleotide and a dual specificity oligonucleotide composed of three portions of the same. The characteristics of the dual specificity oligonucleotide, which are mismatch tolerance and high hybridization specificity, are demonstrated in the present invention.

机译：合成核酸分子，选择性地扩增DNA或核酸混合物的靶核酸序列，同时扩增两个或多个靶核苷酸序列，对靶核酸分子进行测序以检测遗传多样性核酸分子的方法用于检测核酸样品中的靶核苷酸序列，并允许通过寡核苷酸结构和双特异性寡核苷酸确定寡核苷酸的环状特异性。本发明涉及使用双特异性寡核苷酸和由其三个部分组成的双特异性寡核苷酸进行模板依赖性延伸反应的各种方法。在本发明中证实了双重特异性寡核苷酸的错配耐受性和高杂交特异性的特征。

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公开/公告号BRPI0609031B1

专利类型
公开/公告日2017-09-26

原文格式PDF
申请/专利权人 SEEGENE INC.;
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申请/专利号BR2006PI09031
发明设计人 JONG-YOON CHUN;
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申请日2006-03-03
分类号C12N15/11;C12Q1/68;
国家 BR
入库时间 2022-08-21 13:39:46

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