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methods for producing a mutant plant and for generating a plant, plant, seed, rna, methods for producing a cell, for duplicating a gene fragment, for replacing a first promoter sequence, for inserting a regulatory element in a nucleotide sequence, and for inserting an intron in a nucleotide sequence, maize plant and plant cell
methods for producing a mutant plant and for generating a plant, plant, seed, rna, methods for producing a cell, for duplicating a gene fragment, for replacing a first promoter sequence, for inserting a regulatory element in a nucleotide sequence, and for inserting an intron in a nucleotide sequence, maize plant and plant cell
The present disclosure includes the production of a mutant plant resistant to a phosphonomethyl glycine herbicide family, for example glyphosate. Compositions and methods for editing a nucleotide sequence of interest in a cell employing the cas rna guide / endonuclease system are provided, wherein the cas endonuclease is guided by a guide polynucleotide to recognize and optionally introduce a double stranded break. at a specific target site in the genome of a cell. the nucleotide sequence of interest to be edited may be located within or outside the target site that is recognized by an endonuclease cas. More specifically, compositions and methods for editing an enolpyruvylchikimate-3-phosphate synthase (epsps) nucleotide sequence in a cell are provided. The methods and compositions employ a cas / endonuclease polynucleotide system to provide an effective system for editing epsps nucleotide sequences within the genome of a cell. Compositions and methods for producing glyphosate tolerant plant cells, plant explants, seeds and grains are also provided.
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