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METHOD FOR EVALUATING SUITABILITY OF DUODENAL FLUID SAMPLE AS SAMPLE FOR DETECTING PANCREATIC FLUID-DERIVED COMPONENTS
METHOD FOR EVALUATING SUITABILITY OF DUODENAL FLUID SAMPLE AS SAMPLE FOR DETECTING PANCREATIC FLUID-DERIVED COMPONENTS
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机译:评估十二指肠样本的适宜性作为检测胰液衍生成分的样本的方法
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摘要
A method is provided for evaluating the suitability of a duodenal fluid sample collected from an animal as a sample for detecting pancreatic fluid-derived components. The method comprises: (a) mixing a duodenal fluid sample with a chymotrypsin-specific substrate and measuring an amount of degradation the chymotrypsin-specific substrate by of the duodenal fluid sample, (b) mixing the duodenal fluid sample with a pepsin-specific substrate and measuring an amount of degradation of the pepsin-specific substrate by the duodenal fluid sample, and (c) evaluating that the duodenal fluid sample is suitable as a sample for detecting pancreatic fluid-derived components if the amount of degradation of the chymotrypsin-specific substrate by the duodenal fluid sample is higher than a prescribed threshold value and the amount of degradation of the pepsin-specific substrate by the duodenal fluid sample is lower than a prescribed threshold value, as being suitable as a sample for detecting pancreatic fluid-derived components.
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