• 主题
高级搜索  >
历史搜索 清空历史
首页> 外国专利> UNIVERSAL, GLYCOSYLATION ENHANCER, COMPLETELY CHEMICALLY DEFINED MEDIUM FORMULATION

UNIVERSAL, GLYCOSYLATION ENHANCER, COMPLETELY CHEMICALLY DEFINED MEDIUM FORMULATION

机译：通用，糖基化增强剂，完全化学定义的中型配方

页面导航
  • 摘要
  • 著录项
  • 相似文献

摘要

In one embodiment, the present application discloses a cell culture medium for culturing cell lines suitable for producing a therapeutic protein, comprising an amino acid selected from a group consisting of L-arginine, L-asparagine, L-proline, L leucine and L hydroxyproline and a mixture thereof; a vitamin selected from a group consisting of ascorbic acid Mg2+ salt, biotin, pyridoxine HCL, folic acid, riboflavin and D-calcium pantothenate, and a mixture thereof; an element selected from a group consisting of ammonium meta vanadate, sodium meta vanadate, germanium dioxide, barium acetate, aluminum chloride, rubidium chloride, cadmium chloride, ammonium molybedate, stannous chloride, cobalt chloride, chromium sulfate, silver nitrate, sodium metasilicate, zinc sulfate, manganese sulfate H2O, manganous chloride, ferric nitrate 9H2O, ferrous sulfate 7H2O, ferric ammonium citrate, magnesium chloride anhydrous, and magnesium sulfate anhydrous, and a mixture thereof; a nucleoside selected from a group consisting of uridine and cystidine; a sugar selected from a group consisting of galactose, mannose and N-Acetyl-D-Mannosamine; and a triple buffering system comprising sodium carbonate, sodium bicarbonate and HEPES; wherein the cell culture medium is animal component-free, plant component-free, serum-free, growth factors-free, recombinant protein-free, lipid-free, steroid-free, and free of plant or animal hydrolysates and/or extracts.
机译：在一个实施方案中，本申请公开了一种用于培养适于产生治疗性蛋白质的细胞系的细胞培养基，其包含选自L-精氨酸，L-天冬酰胺，L-脯氨酸，L亮氨酸和L羟脯氨酸的氨基酸。及其混合物;维生素，选自抗坏血酸Mg 2 + 盐，生物素，吡ido醇盐酸盐，叶酸，核黄素和D-泛酸钙，及其混合物。元素选自偏钒酸铵，偏钒酸钠，二氧化锗，乙酸钡，氯化铝，氯化rub，氯化镉，钼酸铵，氯化亚锡，氯化钴，硫酸铬，硝酸银，偏硅酸钠，锌硫酸盐，硫酸锰H 2 O，氯化锰，硝酸铁9H 2 O，硫酸亚铁7H 2 O，柠檬酸铁铵，氯化镁无水和无水硫酸镁及其混合物;选自尿苷和胱氨酸的核苷;选自半乳糖，甘露糖和N-乙酰-D-甘露糖胺的糖;包括碳酸钠，碳酸氢钠和HEPES的三重缓冲系统;其中所述细胞培养基不含动物成分，不含植物成分，不含血清，不含生长因子，不含重组蛋白，不含脂质，不含类固醇，并且不含植物或动物水解产物和/或提取物。

著录项

相似文献

  • 专利
  • 外文文献
  • 中文文献
获取专利

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号