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5-oxoprolinase, 5-oxoprolinase gene, and method for producing 5-oxoprolinase
5-oxoprolinase, 5-oxoprolinase gene, and method for producing 5-oxoprolinase
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机译：5-氧代脯氨酸酶，5-氧代脯氨酸酶基因以及产生5-氧代脯氨酸酶的方法
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摘要
Provided is a 5-oxoprolinase that can be suitably used for applications such as improving the umami taste of foods and beverages, separating optical isomers from racemates, and quantifying L-pyroglutamic acid. A 5-oxoprolinase derived from a microorganism belonging to the genus Aspergillus, and a 5-oxoprolinase gene encoding it. A microorganism containing the 5-oxoprolinase gene or a microorganism capable of producing 5-oxoprolinase can be cultured in a medium, and the 5-oxoprolinase of the present invention can be collected from the resulting culture. 5-oxoprolinase is less susceptible to changes in pH than known enzymes, can be used in a wide range of pH, works well under high-temperature, medium-temperature conditions, and has high affinity with substrates Can be provided. In addition, since the 5-oxoprolinase of the present invention is derived from the genus Aspergillus having a long dietary experience, it has an advantage that it is highly safe for use in processing foods and drinks.
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