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A method for localized in situ detection of mRNA

机译:一种mRNA的局部原位检测方法

摘要

The present invention relates to the detection of RNA in a sample of cells. More particularly, the present invention relates to the localized detection of RNA in situ. The method relies on the conversion of RNA to complementary DNA prior to the targeting of the cDNA with a padlock probe(s). The hybridization of the padlock probe(s) relies on the nucleotide sequence of the cDNA which is derived from the corresponding nucleotide sequence of the target RNA. Rolling circle amplification of the subsequently circularized padlock probe produces a rolling circle product which may be detected. Advantageously, this allows the RNA to be detected in situ.
机译:本发明涉及细胞样品中RNA的检测。更具体地,本发明涉及原位RNA的局部检测。该方法依靠在用挂锁探针靶向cDNA之前将RNA转化为互补DNA。挂锁探针的杂交依赖于cDNA的核苷酸序列,其来源于靶RNA的相应核苷酸序列。随后圆形的挂锁探针的滚环放大产生了可以检测到的滚环积。有利地,这允许在原位检测RNA。

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