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METHOD FOR GENERATING HIGH-TITER HEPATITIS E VIRUS STOCKS, AND ASSAY FOR TITRATION OF HEPATITIS E VIRUS
METHOD FOR GENERATING HIGH-TITER HEPATITIS E VIRUS STOCKS, AND ASSAY FOR TITRATION OF HEPATITIS E VIRUS
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机译:产生较高滴度肝炎病毒的方法以及滴定肝炎病毒的评估方法
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摘要
PROBLEM TO BE SOLVED: To provide a method for generating high-titer hepatitis E virus stocks, and to provide an assay for titration of hepatitis E virus.SOLUTION: This invention provides: the use of polybrene as an additive in cell culture medium, used in methods for the generation of high-titer hepatitis E virus stocks; and assays for titration of hepatitis E virus. This invention further provides: a cell culture medium containing polybrene, used for high-titer HEV generation; a method for determining the presence and/or the level of HEV in a sample; and an HEV titration assay using the polybrene.SELECTED DRAWING: None
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