首页> 外国专利> SELECTION OF EXTRACELLULAR MATRIX COMPONENTS AND/OR MATRICELLULAR PROTEINS FOR IMPROVED POST-CRYOPRESERVATION CELL VIABILITY AND RETENTION

SELECTION OF EXTRACELLULAR MATRIX COMPONENTS AND/OR MATRICELLULAR PROTEINS FOR IMPROVED POST-CRYOPRESERVATION CELL VIABILITY AND RETENTION

机译:选择细胞外基质成分和/或细胞胶质蛋白以改善冷冻保存后细胞的活力和保留能力

摘要

A method for cryopreservation of adherent cells attached to a substrate and a method for identifying one or more extracellular matrix (ECM) components and/or matricellular proteins that improves viability and retention of the cryopreserved cells on the substrate. The method for cryopreservation includes treating a substrate with at least one ECM component and/or adding at least one matricellular protein to the substrate or cell culture media, plating the cells on the treated substrate, and cryopreserving the cells. One or more ECM components and/or matricellular proteins that improves cell viability and retention can be identified by evaluating the cells that have been thawed from the cryopreservation temperature to determine cell viability and retention.
机译:一种用于冷冻保存附着在基质上的贴壁细胞的方法,以及一种用于识别一种或多种细胞外基质(ECM)成分和/或基质细胞蛋白的方法,该方法可以提高冷冻保存的细胞在基质上的存活率和保留率。冷冻保存的方法包括用至少一种ECM组分处理基质和/或向基质或细胞培养基中添加至少一种基质细胞蛋白,将细胞铺板在处理过的基质上,并冷冻保存细胞。可以通过评估已经从低温保存温度解冻的细胞以确定细胞生存力和保留能力来鉴定一种或多种改善细胞生存能力和保留能力的ECM成分和/或母细胞蛋白。

著录项

  • 公开/公告号EP3209127A1

    专利类型

  • 公开/公告日2017-08-30

    原文格式PDF

  • 申请/专利权人 LIFELINE SCIENTIFIC INC.;

    申请/专利号EP20150797498

  • 发明设计人 BROCKBANK KELVIN G.M.;CAMPBELL LIA;

    申请日2015-10-26

  • 分类号A01N1/02;A61L27/36;

  • 国家 EP

  • 入库时间 2022-08-21 14:03:52

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