首页> 外国专利> USE OF A GLOBIN, A GLOBIN PROTOMER OR AN EXTRACELLULAR HEMOGLOBIN FOR THE PRESERVATION OF ORGANS, TISSUES, ORGAN AND TISSUE CELLS

USE OF A GLOBIN, A GLOBIN PROTOMER OR AN EXTRACELLULAR HEMOGLOBIN FOR THE PRESERVATION OF ORGANS, TISSUES, ORGAN AND TISSUE CELLS

机译:球蛋白,球蛋白原或细胞外血红蛋白在保存器官,组织,器官和组织细胞中的用途

摘要

Use of a globin and/or globin protomer and/or a native hemoglobin, naturally extra cellular, invertebrate animal comprising phylum Annelida, belonging to marine worms comprising Arenicola marina, at a concentration of 0.625-100 mg/ml, preferably 1.25 mg/ml, in combination with organ preservative solution, or a cell culture medium, for constituting a composition for organ, tissue or cells of organs or tissue preservation, for perfusion of organ or tissues, or for the culture of organ cells or tissues, is claimed. Independent claims are included for: (1) a composition comprising the globin and/or globin protomer and/or the extracellular hemoglobin and the organ preservation solution; (2) a process for preserving the organ or tissue or cells of organ or tissue, preferably organ, comprising rinsing the organ or tissue previously collected, at a temperature of 4-37[deg] C, preferably 4[deg] C, with the composition, and preserving of the organ or tissue by static or dynamic perfusion at a temperature of 4-37[deg] C, preferably 4[deg] C, for a determined time, according to the organ or tissue, in the composition; (3) a process for culturing the organ cells or tissue cells from the organs of vertebrates, comprising culturing the cells, previously collected from vertebrate, at a temperature of 4-37[deg] C, preferably 37[deg] C, for a specified period, depending on the cells, in the composition, and collecting the cells by washing and lysis of the cell mat; and (4) a process for culturing the organ cells or tissue cells from invertebrates, comprising collecting the cells of invertebrate, culturing at a temperature of 4-37[deg] C, preferably 4[deg] C, for a fixed time, depending on the cells in the composition, and collecting the cells by washing and lysis of the cell mat.
机译:球蛋白和/或球蛋白protomer和/或天然血红蛋白的天然外细胞无脊椎动物,其包含浓度为0.625-100mg / ml,优选1.25mg / ml的属于包括海螺的海洋蠕虫的An科的门生动物。本发明涉及与器官防腐溶液或细胞培养基组合的用于构成器官或组织或器官保存的器官,组织或细胞的组合物,用于器官或组织的灌注或用于器官细胞或组织的培养的组合物。包括以下独立权利要求:(1)包含球蛋白和/或球蛋白启动子和/或细胞外血红蛋白和器官保存溶液的组合物; (2)保存器官或组织,优选器官的器官或组织或细胞的方法,包括在4-37℃,优选4℃的温度下漂洗先前收集的器官或组织,所述组合物,并通过在4-37℃,优选4℃的温度下根据器官或组织的静态或动态灌注在器官中确定器官或组织达确定的时间; (3)从脊椎动物器官中培养器官细胞或组织细胞的方法,该方法包括在4-37℃,优选37℃的温度下培养先前从脊椎动物中收集的细胞。特定的时间,取决于细胞,在组成中,并通过洗涤和裂解细胞垫收集细胞; (4)从无脊椎动物培养器官细胞或组织细胞的方法,包括收集无脊椎动物细胞,在4-37℃,优选4℃的温度下培养固定时间,这取决于在组合物中的细胞上沉淀,并通过洗涤和裂解细胞垫收集细胞。

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