首页> 外国专利> ANALOGUES OF NATURAL DEOXYRIBONUCLEOSIDE TRIPHOSPHATES AND RIBONUCLEOSIDE TRIPHOSPHATES CONTAINING REPORTER FLUORESCENT GROUPS, FOR USE IN ANALYTICAL BIOORGANIC CHEMISTRY

ANALOGUES OF NATURAL DEOXYRIBONUCLEOSIDE TRIPHOSPHATES AND RIBONUCLEOSIDE TRIPHOSPHATES CONTAINING REPORTER FLUORESCENT GROUPS, FOR USE IN ANALYTICAL BIOORGANIC CHEMISTRY

机译:包含报告荧光团的天然脱氧核糖核苷三磷酸和核糖核苷三磷酸的类似物,用于分析型生物有机化学

摘要

FIELD: chemistry.;SUBSTANCE: present invention relates to analytical bioorganic chemistry. Disclosed fluorescence-labelled dNTP and rybonucleozidtriphosfates have general formula H-L-F, where H is modified at final phosphorus atom natural dNTP or rybonucleozidtriphosfate, L is linker group, connected to final phosphorus atom and constructed on basis of secondary diamines, F is fluorescent reporter group, connected to linker by means of secondary amine groups. Method for synthesis of fluorescent-labelled nucleotides includes connection of the linker to fluorofor, activation triphosfate groups by converting them in cyclic anhydride shape, interaction of obtaining activated with dyes containing linker group, and purification of desired compounds.;EFFECT: disclosed fluorescence-labelled nucleotides can be used in methods of single molecular sequencing, expanding range of possible conditions of its performance.;4 cl, 4 dwg, 1 tbl, 8 ex
机译:技术领域本发明涉及分析生物有机化学。公开的荧光标记的dNTP和rybonucleozidtriphosfate具有通式HLF,其中H在最终的磷原子上被修饰天然dNTP或rybonucleozidtriphosfate,L是连接基团,连接到最终的磷原子上并基于仲二胺构造,F是荧光报告基团,已连接通过仲胺基与连接基连接。合成荧光标记核苷酸的方法包括:将连接基连接至氟,连接三氟酸酯基团,将其转变成环酐形状,将活化的三氟膦酸酯基团与含有连接基团的染料相互作用,以及纯化所需化合物。核苷酸可用于单分子测序的方法中,扩大了其性能可能条件的范围。; 4 cl,4 dwg,1 tbl,8 ex

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