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PRODUCTION OF THERMOLYSINE AND ITS OPTIONS AND ITS USE IN LIQUID DETERGENTS
PRODUCTION OF THERMOLYSINE AND ITS OPTIONS AND ITS USE IN LIQUID DETERGENTS
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机译：热赖氨酸的生产及其选择及其在液体洗涤剂中的用途
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摘要
1. An isolated thermolysin variant, wherein said thermolysin variant comprises a substitution selected from T006A, T006C, T049D, Y151S, T049M, I156E, T049S, I156H, A056C, I156K, A056R, A056Y, A058S, S065C, S665, G196656 S065T Q273A S065V Q273N Q273T Q128C Q273W T278C Q128T T278H T278N Y151A T278S Y151C T278Y , A061E, Q061M, S065D, S65P of the amino acid sequence shown in SEQ ID NO: 3, moreover, the isolated variant of thermolysin has increased stability compared to wild-type thermolysin. 2. The selected thermolysin variant according to claim 1, wherein said thermolysin variant is a thermolysin variant of the microorganism Geobacillus sp. 3. The isolated thermolysin variant according to claim 1, wherein said Geobacillus microorganism is G. caldoproteolyticus or G. stearothermophilus. 4. The selected thermolysin variant according to claim 1, wherein said thermolysin variant is a thermolysin variant of the microorganism Geobacillus caldoproteolyticus. 5. The isolated variant of thermolysin according to claim 1, wherein said substitution is selected for a substitution equivalent to positions 6, 49, 58, 128, 151, 156, 196, 273, 278 and 280 of the amino acid sequence shown in SEQ ID NO: 3.6. The selected thermolysin variant according to claim 5, wherein said substitution is selected from T006A, T006C, T049D, Y151S, T049M, I156E, T049S, I156H, A056C, I156K, A056R, A056Y, A058S, S065C, S065E19665196 Q273A S065V Q273N Q273T Q128C Q273W T278C Q128T T278H T278N Y151A T278S Y151C T278Y , Q061M, S065D, S65P. 7. A cleaning composition containing an isolated variant of thermolysin according to claim 1 and a detergent. The cleaning composition of claim 7, further comprising a neutral metalloprotease inhibitor. The cleaning composition of claim 7, wherein said neutral metalloprotease inhibitor is galardin. The cleaning composition of claim 7, wherein galardin stabilizes or increases the effectiveness of thermolysin. The cleaning composition of claim 7, wherein said thermolysin variant is a thermolysin variant of the Geobacillus sp.12 microorganism. The cleaning composition according to claim 7, wherein said Geobacillus microorganism is G. caldoproteolyticus or G. stearothermophilus. 13. The cleaning composition of claim 12, wherein said variant thermolysin is a thermolysin variant of the microorganism G. caldoproteolyticus. Chis
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