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epithelialmesenchymal transition EMT Cell culture substrate for inducing epithelialmesenchymal transition use thereof and preparing the same

机译:上皮间质转化EMT细胞培养基质,用于诱导其上皮间质转化的用途及其制备方法

摘要

The present invention relates to a cell culture substrate for epithelial-mesenchymal transition (EMT) induction, wherein a columnar structure having a certain average diameter including a flat surface on which cells are adhered and cultured on the upper part, Wherein the columnar structure is controlled to have a distance from the center of one column to the center of the adjacent column of 4 占 퐉 to 30 占 퐉; A drug screening method using the cell culture substrate; A method for screening a patient-customized anticancer agent and fibrosing agent comprising the step of treating the cell culture substrate with cells isolated from the cancer tissue of the patient by treating or non-treating the anticancer agent candidate substance; And a step of preparing an intermediate mold comprising a plurality of columnar structures comprising an intaglio-patterned elastic polymer.
机译:本发明涉及用于上皮-间质转化(EMT)诱导的细胞培养基质,其中具有一定平均直径的柱状结构包括在其上部分上粘附和培养细胞的平坦表面,其中所述柱状结构被控制从一列的中心到相邻列的中心的距离为4占퐉至30占퐉;一种使用细胞培养底物的药物筛选方法;筛选患者定制的抗癌剂和纤维化剂的方法,包括以下步骤:通过治疗或不治疗抗癌剂候选物质,用从患者的癌组织中分离的细胞处理细胞培养底物;以及制备中间模具的步骤,该中间模具包括多个包含凹版图案化的弹性聚合物的柱状结构。

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