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CLC-Ka Cell line for cell-based high-throughput screening of CLC-Ka channel modulators using YFP fluorescence imaging and broad-spectrum studies of CLC-Ka channels
CLC-Ka Cell line for cell-based high-throughput screening of CLC-Ka channel modulators using YFP fluorescence imaging and broad-spectrum studies of CLC-Ka channels
The present invention relates to a cell line transformed with a plasmid comprising a gene coding for a CLC-Ka channel, a gene coding for Barttin (Y98A) and a gene coding for a fluorescent protein, wherein the CLC-Ka channel and Barttin (Y98A) Is characterized in that it is epitope tagged at the N-terminal or C-terminal and that the fluorescent protein reacts with the halide ion in yellow and stably expresses the CLC-Ka channel, Barttin (Y98A) and fluorescent protein . In addition, it has an inducible expression system expressed by tetracycline induction, can be used for high-efficiency retrieval using cell-based fluorescence imaging, and stably expresses CLC-Ka channel and Barttin (Y98A) In addition, the present invention can be used not only for studying the intracellular signal transduction process involved in the present invention, but also for studying the molecular level of the CLC-Ka channel mechanism using the cell line according to the present invention, studying physiological function, Can be widely used for the development of preventive and therapeutic agents for related kidney and heart diseases.
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