Methods for Simultaneously Detecting Vancomycin-Resistant Enterococci and Kits Using the Same

• 1. 抗万古霉素肠球菌的引物对及检测试剂盒和检测方法 [P] . 中国专利： CN106520921A . 2017-03-22
• 2. 一种耐万古霉素肠球菌的多重荧光定量PCR检测试剂盒及其检测方法 [P] . 中国专利： CN103993089B . 2016.05.11
• 3. Methods for Simultaneously Detecting Vancomycin-Resistant Enterococci and Kits Using the Same [P] . 韩国专利： KR101614916B1 . 2016-04-22

  机译： 同时检测耐万古霉素的肠球菌的方法和使用该试剂盒的试剂盒

• 4. Methods for Simultaneously Detecting Vancomycin-Resistant Enterococci and Kits Using the Same [P] . 韩国专利： KR101614918B1 . 2016-04-22

  机译： 同时检测耐万古霉素的肠球菌的方法和使用该试剂盒的试剂盒

• 5. METHODS FOR SIMULTANEOUSLY DETECTING VANCOMYCIN-RESISTANT ENTEROCOCCI AND KITS USING THE SAME [P] . 韩国专利： KR20150113786A . 2015-10-08

  机译： 相同方法同时检测耐万古霉素的肠球菌和试剂盒的方法

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• 1. Application of H-Point Standard Addition Method and Multivariate Calibration Methods to the Simultaneous Kinetic-Potentiometric Determination of Cerium(IV) and Dichoromate [J] . Mohammad Ali Karimi ,Mohammad Hossein Mashhadizadeh ,Mohammad Mazloum-Ardakani . 美国分析化学（英文） . 2010,第2期
• 2. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 3. DNA extraction from paraffin embedded colorectal carcinoma samples: A comparison study of manual vs automated methods,using four commercially kits [J] . Zsolt Kovacs ,Ioan Jung ,Erzsebet Csernak . 世界临床肿瘤学杂志：英文版 . 2019,第9期
• 4. 258株肠球菌耐药性分析及耐万古霉素基因检测 [C] . 马均 ,褚美玲 ,刘海洋 . 第五届全国微生物耐药监测与临床耐药菌感染治疗专题学术会议 . 2012
• 5. 猪链球菌多重PCR检测方法的建立及试剂盒研制 [A] . 马烨 . 2017