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METHOD FOR RAPIDLY AND QUANTATIVELY ANALYZING BETA-LACTAM ANTIBIOTIC RESISTANCE AND METHOD FOR EVALUATING RESISTANCE USING THE SAME
METHOD FOR RAPIDLY AND QUANTATIVELY ANALYZING BETA-LACTAM ANTIBIOTIC RESISTANCE AND METHOD FOR EVALUATING RESISTANCE USING THE SAME
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机译:快速定量分析β-内酰胺抗药性的方法和使用相同方法评估抗药性的方法
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摘要
The present invention relates to a method for analyzing beta-lactam antibiotic resistance comprising: (a) a pretreatment step including a step of culturing a strain resistant to beta-lactam antibiotics and a strain susceptible to the antibiotics, a step of exposing the cultured strains to the beta-lactam antibiotics, and a step of filtering the resultant product; and (b) measuring resistance against the beta-lactam antibiotics of the pretreated strains by using LC-MS/MS in which two mass spectrometers (MS) are connected to a liquid chromatography (LC). According to the present invention, the method for analyzing beta-lactam antibiotic resistance can quantify antibiotic resistance, and measure the resistance in 2 hours using the simple pretreatment method.
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