首页> 外国专利> MEDIUM COMPOSITION FOR CULTURING FIBROBLAST, METHOD FOR CULTURING FIBROBLAST, AND SKIN REGENERATION COMPOSITION USING METHOD

MEDIUM COMPOSITION FOR CULTURING FIBROBLAST, METHOD FOR CULTURING FIBROBLAST, AND SKIN REGENERATION COMPOSITION USING METHOD

机译:用于培养成纤维细胞的培养基组合物,用于培养成纤维细胞的方法以及使用该方法的皮肤再生组合物

摘要

The present invention relates to a medium composition for culturing fibroblast, a method for culturing fibroblast using the medium, and a skin regeneration composition comprising the cultured fibroblast. More specifically, the present invention relates to a method for culturing fibroblast, which comprises the following steps: preparing fibroblast; culturing fibroblast on a culture plate coated with an extracellular matrix by using a culture fluid comprising a serum substitute; and separating the cultured fibroblast. The present invention further relates to a skin regeneration composition using the same. The medium composition for culturing fibroblast and the method for culturing fibroblast using the same can promote proliferation of cells and inhibits cell aging without uses of the serum. In addition, culturing fibroblast which retains normal cell conformation even for a long period of culturing time is also possible as well. The fibroblast cultured thereby can be useful as the skin regeneration composition and a skin anti-aging composition.;COPYRIGHT KIPO 2016
机译:本发明涉及用于培养成纤维细胞的培养基组合物,使用该培养基培养成纤维细胞的方法以及包含该培养的成纤维细胞的皮肤再生组合物。更具体地,本发明涉及培养成纤维细胞的方法,其包括以下步骤:制备成纤维细胞;和通过使用包含血清替代物的培养液,在涂有细胞外基质的培养板上培养成纤维细胞;并分离培养的成纤维细胞。本发明进一步涉及使用皮肤再生组合物的皮肤再生组合物。用于培养成纤维细胞的培养基组合物和使用该培养基组合物的培养方法可以在不使用血清的情况下促进细胞增殖并抑制细胞衰老。另外,也可以培养即使长时间培养也能保持正常细胞构象的成纤维细胞。由此培养的成纤维细胞可用作皮肤再生组合物和皮肤抗衰老组合物。; COPYRIGHT KIPO 2016

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