首页> 外国专利> A 3D POROUS CELLULOSE SCAFFOLD FOR BONE TISSUE ENGINEERING AND THE METHOD FOR ITS PREPARATION

A 3D POROUS CELLULOSE SCAFFOLD FOR BONE TISSUE ENGINEERING AND THE METHOD FOR ITS PREPARATION

机译:用于骨组织工程的3d多孔纤维素支架及其制备方法

摘要

The present invention relates to material engineering and could be applied in odontology or other kind of surgery for bone tissue regeneration in a defect site. The aim of this invention is to prepare a porous three dimensional cellulose-based biocompatible scaffold of good osteoconductive and mechanical properties with the structure like that of a natural bone. To achieve the above object, the present invention provides a composite comprising cellulose and biogenic bone particles at a ratio (w/w) 1 :0.12-6.0, wherein the diameter of the particles is 0.01-2000 microns. In a separate case a bone material which comprises inorganic compounds and polysaccharide chitin could be used as a biogenic bone. In a separate case the surface of the scaffold could be coated with collagen, platelet- rich fibrin, various growth factors, therapeutic additives, stem cells. The three dimensional porous scaffold of the natural bone morphology is prepared by inserting biogenic bone particles into a cellulose gel during its formation from cellulose acetate at a ratio 1 :0.12- 6.0, afterwards loading the gel with an aqueous 10-30 % ethanol solution and freeze-dried or extracted with carbon dioxide at supercritical conditions at 24-70 MPa and 35-80°C temperature. The resulting scaffold could be ground to granules of a desired size and/or the pasta could be prepared.
机译:本发明涉及材料工程,并且可以应用于牙科学或其他类型的手术中以在缺损部位进行骨组织再生。本发明的目的是制备具有良好的骨传导性和机械性能并具有类似于天然骨的结构的多孔的三维纤维素基生物相容性支架。为实现上述目的,本发明提供了一种复合材料,其包含纤维素和生物骨颗粒的比例(w / w)为1:0.12-6.0,其中颗粒的直径为0.01-2000微米。在单独的情况下,包含无机化合物和多糖几丁质的骨材料可以用作生物骨。在单独的情况下,支架的表面可以涂有胶原蛋白,富含血小板的纤维蛋白,各种生长因子,治疗性添加剂,干细胞。天然骨形态的三维多孔支架的制备方法是,在形成过程中将生物源性骨颗粒以1:0.12-6.0的比例从醋酸纤维素中插入纤维素凝胶中,然后在凝胶中加入10-30%的乙醇水溶液和在24-70 MPa和35-80°C温度下,在超临界条件下冷冻干燥或用二氧化碳萃取。可以将所得支架研磨成所需大小的颗粒和/或可以制备面食。

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