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MONOCLONAL ANTIBODY BASED ONLINE PHOSPHOPROTEIN PROTEOMICS ANALYSIS METHOD USING MICROBORE HOLLOW FIBER ENZYMATIC REACTOR-TANDEM MASS SPECTROMETRY
MONOCLONAL ANTIBODY BASED ONLINE PHOSPHOPROTEIN PROTEOMICS ANALYSIS METHOD USING MICROBORE HOLLOW FIBER ENZYMATIC REACTOR-TANDEM MASS SPECTROMETRY
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机译：微孔空心纤维酶联反应串联质谱的基于单链抗体的磷酸化蛋白质组学分析方法
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摘要
The present invention relates to a phosphoprotein extraction method and a mass spectrometric method using a microbore hollow fiber enzymatic reactor (mHFER) based antigen-antibody reaction and, specifically, to an extraction method and a mass spectrometric method, wherein phosphoproteins or phosphopeptides present in the body are extracted using phosphoserine-, phosphothreonine-, and phosphotyrosine-antibodies, and measured by a mass spectrometer, and thus biomarker phosphoproteins for diagnosis of diseases are found, contributing to early diagnosis of diseases. The mass spectrometric method using the antigen-antibody reaction based extraction method of the present invention can: minimize temporal and economic burdens resulting from a low extraction rate and a complicated sample pre-treatment; increase the extraction efficiency by using a considerable number of phosphopeptides (or phosphoproteins) and antibodies with strong affinity; and allow the extraction of low-concentration phosphopeptides or phosphoproteins, and thus the present invention is expected to have high applicability in discovering disease diagnosis protein markers and identifying and studying mechanisms thereof.
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