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METHOD OF DETECTING BLOOD PLASMA SCHIZANDRIN B AFTER ADMINISTRATION OF FUZHENG HUAYU (FZHY)
METHOD OF DETECTING BLOOD PLASMA SCHIZANDRIN B AFTER ADMINISTRATION OF FUZHENG HUAYU (FZHY)
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机译：福建华药管理后血浆中五味子B素的检测方法
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摘要
A method of detecting blood plasma schizandrin B after administration ofFuzheng Huayu(FZHY) is disclosed. The method includes: (1) extracting schizandrin B fromplasma of amammal administered FZHY by ethyl acetate with the ratio 1:4, whirling 3-5mins,centrifuging at 9600rpm for mins, drying and enriching the upper layer at 25-30 °C, andredissolving with mobile phase; (2) UPLC/MS measuring wherein the UPLCconditioncomprises an Acquity UPLC BEH C18, 2.1 x 100 mm chromatographic column, mobilephase A comprising water-acetonitrile-formic acid at a ratio (v/v/v) of95:5:0.1, and mobilephase B comprising acetonitrile-formic acid at a ratio (v/v) of 100:0.1; andthe MScondition comprises an electric spraying ion source (ESI) detecting withpositive ion modeand scanning at the range of rn/z 150-1200. The method can be used forpharmacokineticsstudies of schizandrin B after administration of FZHY.
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机译：给药后检测血浆五味子乙素B的方法扶正华宇（FZHY）被公开。该方法包括：（1）从中提取五味子素B。血浆哺乳动物以1：4的比例通过乙酸乙酯施用FZHY，旋转3-5分钟以9600rpm的转速离心几分钟，干燥并在25-30°C，和用流动相重新溶解（2）UPLC / MS测量，其中UPLC健康）状况包括Acquity UPLC BEH C18、2.1 x 100 mm色谱柱，可移动相A（水/乙腈-甲酸）的比例（v / v / v）为95：5：0.1，并且移动B相，其中乙腈-甲酸的比例（v / v）为100：0.1;和MS病情包括电喷雾离子源（ESI）正离子模式并在rn / z 150-1200范围内扫描。该方法可用于药代动力学FZHY后五味子乙素B的研究。
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