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isolation of a bacterial consortium capable of degrading diesel oil and improve the efficiency of the degradation by the addition of a biosurfactant from bacillus subtilis spbl

机译:通过添加枯草芽孢杆菌中的生物表面活性剂,分离出能够降解柴油并提高降解效率的细菌聚生体

摘要

oil contamination is one of the major problems which are harmful to the environment. the use of microorganisms having the ability to damage the hydrocarbons are a quite promising. in this context, we have isolated three strains of bacteria with the ability to damage the diesel.molecular typing showed that it is lysinibacillus bronitolerans ri18 (kf964487); bacillus thuringiensis ri16 (kml 11604) and bacillus weihenstephanensis ir12 (km094930). a lipopeptide biosurfactant which was trying to enhance the biodegradation of diesel by the project.the biodegradation in the presence and absence of 0, 1, biosurafctant of spbl and when co inoculated with bacillus subtilis spbl show improved biodegradation effect of biosurfactant. in fact, an improvement of the biodegradation of some 38.42% and 12.19% in medium liquid and soil contaminated with diesel fuel, respectively, was recorded in presence of 0 1% biosurfactant. also, improvements to the biodegradation of some 49.65 and 15.35% were obtained, respectively, for the water and the soil in the case of the spbl co inoculation with b. subtilis.the analysis of residual diesel oil by gas chromatography is in favour of the gravimetric results. these results suggest the applicability of the project, as well as the biosurfactant of bps for the bioremediation of environments contaminated with diesel fuel.
机译:油污染是对环境有害的主要问题之一。具有破坏碳氢化合物能力的微生物的使用是非常有前途的。在这种情况下,我们分离出了三种具有破坏柴油能力的细菌。分子分型表明,它是细支气管芽孢杆菌ly18(kf964487)。苏云金芽孢杆菌ri16(kml 11604)和魏氏杆菌ir12(km094930)。一种脂肽生物表面活性剂,正在尝试通过该项目提高柴油的生物降解性。在有和无0、1的spbl生物表面活性剂存在下以及与枯草芽孢杆菌spbl一起接种时的生物降解都显示出改善的生物表面活性剂生物降解作用。实际上,在存在0 1%生物表面活性剂的情况下,在中等液体和被柴油污染的土壤中,生物降解率分别提高了约38.42%和12.19%。同样,在spbl与b一起接种的情况下,水和土壤的生物降解率分别提高了约49.65和15.35%。枯草杆菌。通过气相色谱法分析残留柴油有利于重量分析法。这些结果表明该项目的适用性,以及bps的生物表面活性剂对被柴油污染的环境进行生物修复的适用性。

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