首页> 外国专利> Signaling peptide enterotoxin II of E. coli modified and a microorganism expressing a fusion protein of the peptide and a heterologous protein.

Signaling peptide enterotoxin II of E. coli modified and a microorganism expressing a fusion protein of the peptide and a heterologous protein.

机译:修饰了大肠杆菌的信号肽肠毒素II,并表达了该肽和异源蛋白融合蛋白的微生物。

摘要

"Beacon of E.coli enterotoxin II peptide modified and a microorganism expressing a fusion protein of the peptide and a heterologous protein. A heterologous protein is produced by: (I) the cultivation of a microorganism transformed with a vector comprising a gene coding a peptide expression marker of enterotoxin II B.Coli fused to heterologous protein modified to produce and secrete the heterologous protein to periplasma, the peptide marker of enterotoxin II b.coli modified is obtained by substitution of at least one of the second, fourth, fifth, tenth second, the twentieth and twenty sec... The amino acids of the signaling peptide enterotoxin II B.Coli in the following sequence of amino acid (SRQ ID: 1) with another amino acid, provided that at least one of the second and fourth amino acids, peptide modified is lysine; and (II) recovering the heterologous protein of periplasma.
机译:修饰了大肠杆菌肠毒素II肽的信标和表达该肽与异源蛋白的融合蛋白的微生物。异源蛋白是通过以下方法生产的:(I)培养用包含编码Aβ1基因的载体转化的微生物肠毒素II B.coli的肽表达标记融合到经修饰的异源蛋白上,以产生异种蛋白并将其分泌到周质中,肠毒素II b.coli的肽标记通过取代第二,第四,第五,第十秒,第二十秒和第二十秒。信号肽肠毒素II B.Coli的氨基酸,在以下氨基酸序列(SRQ ID:1)中与另一个氨基酸结合,条件是第二个和第二个第四氨基酸,修饰的肽是赖氨酸;和(II)回收周质的异源蛋白。

著录项

相似文献

  • 专利
  • 外文文献
  • 中文文献
获取专利

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号