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MEDIUM, METHOD AND SYSTEM FOR CULTIVATION OF CHLORELLA PYRENOIDOSA OR ORGANISMS DERIVED FROM CHLORELLA PYRENOIDOSA
MEDIUM, METHOD AND SYSTEM FOR CULTIVATION OF CHLORELLA PYRENOIDOSA OR ORGANISMS DERIVED FROM CHLORELLA PYRENOIDOSA
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机译：培养小叶绿藻或来源于小叶绿藻的微生物的培养基，方法和系统
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摘要
The present invention is concerned a method for cultivation of Chlorella pyrenoidosa or organisms derived from Chlorella pyrenoidosa, comprising steps of a) providing a fermenter containing primarily a food waste hydrolysate medium, b) at the beginning of a first phase, introducing a strain of Chlorella pyrenoidosa or Chlorella pyrenoidosa derived organisms in the food waste hydrolysate medium for cultivation, wherein the food waste hydrolysate medium in the first phase contains substantially 5-30 g L−1 glucose, 74-144 mg L−1 free amino nitrogen and 40-64 mg L−1 phosphate, c) during the first phase, allowing diminishing of free amino nitrogen and/or phosphate in the food waste hydrolysate medium until the free amino nitrogen and/or phosphate becomes limited, and d) at the beginning of a second phase after the first phase, feeding a fresh supply of food waste hydrolysate medium in the fermenter, wherein the fresh supply of food waste hydrolysate contains substantially 30-90 g L−1 glucose, 160-230 mg L−1 free amino nitrogen and 80-270 mg L−1 phosphate, and feeding occurs at a certain dilution rate.
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机译：本发明涉及培养小球藻小球藻或源自小球藻小球藻的生物的方法，其包括以下步骤：a）提供主要包含食物废水解产物的发酵罐，b ）在第一阶段开始时，在食物残渣水解产物培养基中引入源自 Chlorella pyrenoidosa 或 Pirenoidosa 来源的生物菌株进行培养，其中第一相基本上包含5-30 g L -1 葡萄糖，74-144 mg L -1 游离氨基氮和40-64 mg L -1 < / Sup>磷酸盐，c）在第一阶段中，允许食物垃圾水解产物介质中的游离氨基氮和/或磷酸盐减少，直到游离氨基氮和/或磷酸盐变得受限为止，和d）在第二阶段开始时在第一阶段之后，在发酵罐中喂入新鲜供应的食物废水解产物培养基，其中新鲜供应的foo d水解产物废料中基本上含有30-90 g L -1 葡萄糖，160-230 mg L -1 游离氨基氮和80-270 mg L -1 ＜ / Sup>磷酸盐，进料以一定的稀释率进行。
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