Protein crystallization analyzer and protein crystallization analysis method

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• 3. PROTEIN CRYSTALLIZATION ANALYZER AND PROTEIN CRYSTALLIZATION ANALYTICAL METHOD [P] . 日本专利： JP2012127904A . 2012-07-05

  机译： 蛋白质结晶分析仪和蛋白质结晶分析方法

• 4. Protein crystallization agent and protein crystallization method using protein crystallization agent [P] . 日本专利： JP5484041B2 . 2014-05-07

  机译： 蛋白质结晶剂及使用该蛋白质结晶剂的蛋白质结晶方法

• 5. protein crystallization method protein crystallization agent and using a protein crystallizing agent [P] . 日本专利： JPWO2008102469A1 . 2010-05-27

  机译： 蛋白质结晶方法蛋白质结晶剂及使用该蛋白质结晶剂

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• 1. Correlation of Combined Characters of Amino Acid and Whole Protein with Success Rate of Crystallization of iLactobacillus/iProteins [J] . Shaomin Yan ,Guang Wu . 生物医学工程（英文） . 2019,第4期
• 2. Prediction of Crystallization Propensity of Proteins from iBacillus haloduran/iUsing Various Amino Acid and Protein Features [J] . Shaomin Yan ,Guang Wu . 生物医学工程（英文） . 2019,第12期
• 3. Correlating Combined Features of Amino Acid and Protein with Crystallization Propensity of Proteins from iMycobacterium tuberculosis/i [J] . Shaomin Yan ,Guang Wu . 生物医学工程（英文） . 2019,第9期
• 4. Sequence-Based Protein Crystallization Propensity Prediction for Structural Genomics: Review and Comparative Analysis [J] . Lukasz Kurgan ,Marcin J. Mizianty . 自然科学期刊（英文） . 2009,第2期
• 5. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 6. 金黄色葡萄球菌噬菌体裂解酶LysGH15催化活性域蛋白质结晶条件筛选 [C] . 夏翡翡 ,顾敬敏 ,冯新 . 第五届中国兽药大会（中国畜牧兽医学会动物药品学分会2014年学术年会、中国畜牧兽医学会生物制药学分会暨中国微生物学会兽医微生物学专业委员会联合学术论坛） . 2014
• 7. 膜结晶法结晶蛋白质的研究 [A] . 张新妙 . 2007