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Process for producing glycoproteins characterized by sugar chain structure using silkworms

机译:用蚕生产具有糖链结构特征的糖蛋白的方法

摘要

It is an object of the present invention to investigate what kinds of structures a sugar chain has by performing a sugar chain structural analysis of a glycoprotein produced in a transgenic silkworm. For the sugar chain of the glycoprotein produced in the transgenic silkworm, a glycoprotein having a sugar chain structure in which fucose is not linked to N-acetylglucosamine at a reducing terminal of the sugar chain is obtained. In addition, a glycoprotein having an almost completely humanized sugar chain structure is obtained by further integrating GalT gene into this transgenic silkworm.
机译:本发明的目的是通过对转基因家蚕中产生的糖蛋白进行糖链结构分析来研究糖链具有什么样的结构。对于在转基因家蚕中产生的糖蛋白的糖链,获得具有糖链结构的糖蛋白,其中岩藻糖未在糖链的还原端与N-乙酰氨基葡糖连接。另外,通过将GalT基因进一步整合到该转基因蚕中,获得具有几乎完全人源化的糖链结构的糖蛋白。

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