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SNP detection by melting curve clustered

机译:通过熔解曲线聚类检测SNP

摘要

Systems, methods and apparatus for an automated analysis of a collection of melt curves is provided. The analysis can identify certain characteristics of double stranded nucleotide sequences (e.g. DNA or other nucleotide sequences) which were melted. For example, a variation (e.g. a mutation) in the sequences (also called amplicons) may be determined from the analysis. The amplicons may be amplified via any amplification mechanism, such as PCR or Ligase chain reaction (LCR). The automated analysis can include identifying a melt region, normalizing a melt curve, and clustering melt curves.
机译:提供了用于自动分析熔解曲线的集合的系统,方法和设备。该分析可以鉴定被融化的双链核苷酸序列(例如DNA或其他核苷酸序列)的某些特征。例如,可以从分析中确定序列(也称为扩增子)的变异(例如突变)。扩增子可以通过任何扩增机制如PCR或连接酶链反应(LCR)来扩增。自动化分析可以包括识别熔体区域,标准化熔体曲线和聚类熔体曲线。

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