首页> 外国专利> METHOD OF SIMULTANEOUS SYNTHESIS OF DNA LIBRARY USING HIGH-THROUGHPUT PARALLEL DNA SYNTHESIS METHOD

METHOD OF SIMULTANEOUS SYNTHESIS OF DNA LIBRARY USING HIGH-THROUGHPUT PARALLEL DNA SYNTHESIS METHOD

机译:高通量并行DNA合成方法同时合成DNA文库的方法

摘要

The method of proposition is readily found mistake, and the gene library after the sequent synthesis for analyzing various library nucleic acid base sequences is applied in combination three nucleic acid base sequences (codon) and translates into identical albumen. It can be seen that the protein sequence system generation having the same of a gene library but different nucleic acid base sequences can be formed. Present disclose provides a kind of novel experimental methods, can measure the correlation between the variation of gene expression, according in expression specific gene body of the use of codon by changing codon optimization. In addition, present disclose provides a kind of simultaneously synthesizing and analyze gene library, wherein protein sequence system is converted into the identical and a kind of gene that the protein sequence system for identifying what change a part is expressed of some similar protein sequence systems generations and affects the corresponding science of heredity of function
机译:提议方法容易被发现是错误的,并且在随后的合成之后用于分析各种文库核酸碱基序列的基因文库被组合使用三个核酸碱基序列(密码子)并翻译成相同的蛋白。可以看出,可以形成具有相同基因库但具有不同核酸碱基序列的蛋白质序列系统。本发明提供了一种新颖的实验方法,可以测量基因表达变化之间的相关性,通过改变密码子的优化来根据表达特定基因体中密码子的使用。另外,本发明提供了一种同时合成和分析基因库的方法,其中蛋白质序列系统被转化为相同的基因,并且该基因序列系统用于识别一些相似的蛋白质序列系统世代中表达什么变化的蛋白质序列系统。并影响相应的功能遗传学

著录项

  • 公开/公告号EP2921560A4

    专利类型

  • 公开/公告日2016-05-04

    原文格式PDF

  • 申请/专利权人 CELEMICS INC.;

    申请/专利号EP20130863345

  • 申请日2013-12-11

  • 分类号C12N15/10;C12N15/67;C40B40/08;C40B50/06;

  • 国家 EP

  • 入库时间 2022-08-21 14:50:48

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