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首页> 外国专利> METHOD OF PREDICTING DEVELOPMENT OF IMMUNE DISORDERS UNDER IMPACT OF STRONTIUM WITH APPLICATION OF IMMUNOGENETIC CRITERIA

METHOD OF PREDICTING DEVELOPMENT OF IMMUNE DISORDERS UNDER IMPACT OF STRONTIUM WITH APPLICATION OF IMMUNOGENETIC CRITERIA

机译：应用免疫遗传标准预测受锶影响的免疫疾病的方法

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FIELD: medicine.;SUBSTANCE: to predict the development of immune disorders in patients living on territories with the high content of strontium in the environment, sampling of blood in the patients from the territory with the higher content of strontium in objects of the environment was carried out. In the said sample determined is the content of strontium in the blood samples of the patients, with the content of strontium higher than the background value, the content of lymphocytes CD8+ is determined. After that, a separated fraction of CD8+ lymphocytes is divided into two parts, one of which is subjected to incubation with strontium, and the second is used as a control one, with the incubation being realised by the introduction of 10 mg/l of strontium into the CD8+ sample. Then, an amount of ribonucleic acid RNA is determined in both samples of CD8+ lymphocytes by its isolation from separated CD8+ cells, performed is a reaction of the reverse RNA transcription into deoxyribonucleic acid DNA, in which the quantity of the alpha defensin gene is determined. In determination the gene of GAPDH household is used as internal control. After that the level of the relative expression of the alpha defensin gene is calculated, taking as a spontaneous expression the level referring to the control CD8+ sample, with the level, referring to the CD8+ sample, into which strontium was introduced, being taken as the induced expression. A conclusion about immune disorders in the patient, associated with the impact of strontium, is made by the index of the expression, which equals the ratio of the induced expression to the spontaneous expression. If the said index is lower than 0.7, the development of an immunodeficiency state is predicted, and if the index is higher than 3, the development of a proliferative status in the immune system, on the condition of the simultaneous detection of higher than 1.5-fold exceedance of strontium in blood in comparison with the background one.;EFFECT: prediction of the immune disorders, associated with the impact of strontium, due to the application of the immunogenetic criteria.;2 tbl, 3 ex
机译：领域：医学;研究对象：为了预测生活在环境中锶含量高的地区的患者的免疫系统疾病的发展，从环境物体中锶含量高的地区的患者中采集血液是执行。在所确定的所述样品中是患者血液样品中的锶含量，当锶含量高于背景值时，测定淋巴细胞CD8 + 的含量。之后，将分离的CD8 + 淋巴细胞分为两部分，其中一部分与锶一起孵育，第二部分用作对照，通过孵育实现。在CD8 + 样品中引入10 mg / l锶。然后，通过从分离的CD8 + 细胞中分离，确定CD8 + 淋巴细胞的两个样品中的核糖核酸RNA的量，这是将RNA逆转录成脱氧核糖核酸DNA，其中确定了α防御素基因的量。在确定中，GAPDH家族的基因用作内部对照。之后，计算α防御素基因的相对表达水平，将参照对照CD8 + 样品的水平作为自发表达，参照CD8 +的水平引入锶的样品为诱导表达。通过表达指数得出与锶影响有关的患者免疫疾病的结论，该指数等于诱导表达与自发表达的比率。如果所述指数低于0.7，则预示着免疫缺陷状态的发展，如果所述指数高于3，则在同时检测到高于1.5-的条件下免疫系统中增殖状态的发展。血中锶的超标倍数与本底相比。效果：由于免疫原性标准的应用，预测了与锶影响有关的免疫疾病。2tbl，3 ex

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