首页> 外国专利> EXPRESSION PLASMID FOR HUMAN RECOMBINANT FOLLICLE-STIMULATING HORMONE (FSH) IN CHO CELLS, EXPRESSION PLASMID FOR BETA-SUBUNIT OF HUMAN RECOMBINANT FSH IN CHO CELLS, CHO CELL PRODUCING HUMAN RECOMBINANT FSH, AND METHOD FOR PRODUCING ABOVE HORMONE

EXPRESSION PLASMID FOR HUMAN RECOMBINANT FOLLICLE-STIMULATING HORMONE (FSH) IN CHO CELLS, EXPRESSION PLASMID FOR BETA-SUBUNIT OF HUMAN RECOMBINANT FSH IN CHO CELLS, CHO CELL PRODUCING HUMAN RECOMBINANT FSH, AND METHOD FOR PRODUCING ABOVE HORMONE

机译：CHO细胞中人重组卵泡刺激素（FSH）的表达质粒，CHO细胞中人重组FSH的β-亚单位的表达质粒，产生人重组FSH的CHO细胞及其生产方法

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摘要

FIELD: medicine.SUBSTANCE: invention refers to biotechnology and can be used for the recombinant production of human follicle-stimulating hormone (FSH). The procedure involves constructing an expression plasmid for human recombinant FSH in CHO cells; the above plasmid contains a region of origin of replication of the pUC plasmid, a beta-lactamase (bla) open reading frame and a bla gene prokaryotic promoter, a human Epstein-Barr virus terminal repeat (EBVTR) site, functional elements of Chinese hamster elongation factor 1 alpha gene, Kozak sequence; open reading frames of alpha- and beta-subunits of human FSH with stop-codons; an internal ribosomal entry site (IRES) of EMCV virus; an open reading frame of dihydrofolate reductase (DHFR) expressed as a part of tricystronic mRNA with the gene coding alpha- and beta-subunit of human FSH. The produced plasmid is used to transform a CHO cell to produce a FSH producer cell. The producer cell can be additionally transformed by the plasmid expressing the beta-subunit of human FSH.EFFECT: invention enables substantially increasing the production effectiveness of human FSH in CHO cells.15 cl, 7 dwg, 3 tbl, 10 ex

机译：技术领域：药物。发明是生物技术，可用于重组人卵泡刺激素（FSH）的生产。该方法涉及在CHO细胞中构建人重组FSH的表达质粒。上述质粒包含pUC质粒的复制起点，β-内酰胺酶（bla）开放阅读框和bla基因原核启动子，人爱泼斯坦-巴尔病毒末端重复序列（EBVTR）位点，中国仓鼠的功能元件延伸因子1α基因，Kozak序列;具有终止密码子的人FSH的α-和β-亚基的开放阅读框; EMCV病毒的内部核糖体进入位点（IRES）;二氢叶酸还原酶（DHFR）的开放阅读框，表达为tricstronic mRNA的一部分，具有编码人FSH的α和β亚基的基因。产生的质粒用于转化CHO细胞以产生FSH产生细胞。产生细胞可以通过表达人FSH的β亚基的质粒另外转化。效果：本发明能够显着提高CHO细胞中人FSH的生产效率。15cl，7 dwg，3 tbl，10 ex

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公开/公告号RU2560596C2

专利类型
公开/公告日2015-08-20

原文格式PDF
申请/专利权人 OBSHCHESTVO S OGRANICHENNOJ OTVETSTVENNOSTJU AJVIFARMA (OOO AJVIFARMA);
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申请/专利号RU20130143210
发明设计人 KOVNIR SERGEJ VLADIMIROVICH;VOROBEV IVAN IVANOVICH;KHODAK JULIJA ALEKSANDROVNA;ORLOVA NADEZHDA ALEKSANDROVNA;
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申请日2013-09-24
分类号C12N15/63;C12N5/10;C12N15/16;C12N15/79;C12P21/02;
国家 RU
入库时间 2022-08-21 14:56:12

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