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METHOD FOR DETECTING TUBERCULOUSIS MYCOBACTERIA OF GENETIC CLUSTER Beijing B0/W148
METHOD FOR DETECTING TUBERCULOUSIS MYCOBACTERIA OF GENETIC CLUSTER Beijing B0/W148
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机译：北京人群B0 / W148基因簇中结核分枝杆菌的检测方法
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摘要
FIELD: medicine.;SUBSTANCE: invention refers to biotechnology, namely to a method for identifying tuberculosis mycobacteria of cluster Beijing B0/W148. A polymerase chain reaction is conducted for amplification of M. tuberculosis DNA fragments with selected primers P1, P2 and P3, sequences of which are presented by SEQ ID NO 1, 2, 3, respectively in the amplification mode of 98°C - 10 minutes, 30 cycles: 98°C - 30 sec, 64°C - 30 sec, 72°C - 50 sec, 72°C - 10 min, 4°C. The amplification products are separated by electrophoresis in 2% agarose gel. If the fragment of 1,021 base pairs is produced, the strain of tuberculosis mycobacteria of cluster Beijing B0/W148 is detected.;EFFECT: presented invention enables fast and high-specificity identification of tuberculosis mycobacteria of cluster Beijing B0/W148.;3 dwg
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机译：技术领域本发明涉及生物技术，即一种鉴定北京B0 / W148簇的结核分枝杆菌的方法。进行聚合酶链式反应以扩增带有选定引物P1，P2和P3的结核分枝杆菌DNA片段，其引物序列分别由SEQ ID NO 1、2、3呈递，其扩增模式为98°C-10分钟30个循环：98°C-30秒，64°C-30秒，72°C-50秒，72°C-10分钟，4°C。通过在2％琼脂糖凝胶中的电泳分离扩增产物。如果产生了1,021个碱基对的片段，则检测到北京B0 / W148簇的结核分枝杆菌菌株。效果：本发明能够快速且高特异性地鉴定北京B0 / W148簇的结核分枝杆菌。3 dwg
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