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Ways to reduce the toxic effect of enrofloxacin on cellular immunity USING IN VITRO homeopathic preparations
Ways to reduce the toxic effect of enrofloxacin on cellular immunity USING IN VITRO homeopathic preparations
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机译:体外使用顺势疗法制剂降低恩诺沙星对细胞免疫毒性作用的方法
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摘要
A method for reducing toxic effects on enrofloxacin cellular immunity using homeopathic preparations in vitro, comprising the use of the leukocyte suspension, antibacterial and homeopathic preparations, diluted in saline dilution, characterized in that a leukocyte suspension in a volume of 0.5 ml was added 0.15 ml antibiotic (Enrofloxacin 5%), then the resulting solution was placed in a thermostat for 3 hours at T = 37 ± 0,5 ° C, after incubation Pipette 0.5 ml of a homeopathic drug isolator ction in saline dilution (C6, 12, 30, C200) and again placed in an incubator for 4 hours at T = 37 ± 0,5 ° C, then making the slurry of microorganisms reference strain of Staphylococcus albus №182 0.5 ml containing 10KOE / in 1 mL of saline, then placed in an incubator for 30 minutes at T = 37 ± 0,5 ° C, after incubation, blood smears fixed in alcohol-formalin 1: 9 (40% formalin solution and 96 alcohol °) for 10 minutes and stained for Romanovskomi - Giemsa for 30 minutes.
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