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Ways to reduce the toxic effect of enrofloxacin on cellular immunity USING IN VITRO homeopathic preparations

机译:体外使用顺势疗法制剂降低恩诺沙星对细胞免疫毒性作用的方法

摘要

A method for reducing toxic effects on enrofloxacin cellular immunity using homeopathic preparations in vitro, comprising the use of the leukocyte suspension, antibacterial and homeopathic preparations, diluted in saline dilution, characterized in that a leukocyte suspension in a volume of 0.5 ml was added 0.15 ml antibiotic (Enrofloxacin 5%), then the resulting solution was placed in a thermostat for 3 hours at T = 37 ± 0,5 ° C, after incubation Pipette 0.5 ml of a homeopathic drug isolator ction in saline dilution (C6, 12, 30, C200) and again placed in an incubator for 4 hours at T = 37 ± 0,5 ° C, then making the slurry of microorganisms reference strain of Staphylococcus albus №182 0.5 ml containing 10KOE / in 1 mL of saline, then placed in an incubator for 30 minutes at T = 37 ± 0,5 ° C, after incubation, blood smears fixed in alcohol-formalin 1: 9 (40% formalin solution and 96 alcohol °) for 10 minutes and stained for Romanovskomi - Giemsa for 30 minutes.
机译:一种体外使用顺势疗法制剂降低对恩诺沙星细胞免疫毒性作用的方法,包括使用稀释于盐水中的白细胞悬液,抗菌和顺势疗法制剂,其特征在于,添加0.5 ml体积的白细胞悬液0.15 ml抗生素(恩诺沙星5%),然后在移液器中吸取0.5 ml生理盐水稀释液中的顺势疗法药物隔离液(C6、12、30)孵育后,将所得溶液在T = 37±0.5°C的恒温器中放置3小时。 ,C200),并再次置于T = 37±0.5°C的培养箱中4个小时,然后使微生物浆液参照白色葡萄球菌№1820.5 ml含10KOE /的1 mL盐水,然后置于孵育器在T = 37±0.5°C下孵育30分钟,孵育后,将血液涂片固定在酒精-福尔马林1:9(40%福尔马林溶液和96酒精度)中10分钟,并用Romanovskomi-Giemsa染色30分钟。

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