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Development of species-specific PCR method for detection of Acinetobacter species
Development of species-specific PCR method for detection of Acinetobacter species
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机译：用于检测不动杆菌种的种特异性PCR方法的开发
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摘要
ahsine Saturday bakteo species of the present invention for detecting primers, probes and using them ahsine Sat It relates to a method for detecting the bakteo species. More particularly, the present invention has a first pair of primers consisting of the nucleotide sequence of SEQ ID NO: 1 and 2; SEQ ID NO: 3 and a second pair of primers consisting of the base sequence of 4; SEQ ID NO: 5, and the third pair of primers consisting of the nucleotide sequence of 6; A fourth pair of primers consisting of the nucleotide sequence of SEQ ID NO: 7 and 8; SEQ ID NO: 9 and 10, a fifth pair of primers consisting of the nucleotide sequence of; A sixth pair of primers consisting of the nucleotide sequence of SEQ ID NO: 11 and 12; The seventh pair of primers consisting of the nucleotide sequence of SEQ ID NO: 13 and 14; SEQ ID NO: 15, and the eighth pair of primers consisting of the nucleotide sequence 16; SEQ ID NO: 17 and 9, 18 of a pair of primers consisting of the base sequence; And SEQ ID NO: 19 and at least one member from the group consisting of the primers consisting of 10 to 20 of the nucleotide sequence comprising a selected primer pair ahsine sat bakteo (Acinetobacter) primers that can specifically detect ahsine species and using the primer Sat relates to a method for detecting bakteo species. Ahsine Saturday bakteo method of detecting ahsine Saturday bakteo using this primer and detection according to the present invention is an infectious hospital Saturday ahsine bakteo knife Core Seti Syracuse ( Acinetobacter calcoaceticus ) , ahsine Saturday bakteo Bauer Mani ( A. baumannii ) , ahsine Saturday bakteo Petey ( A. pittii ) , ahsine au Saturday bakteo Alice Cormier ( A. nosocomialis ),. ahsine Saturday bakteo Earl destination ( A ursingii), ahsine Saturday bakteo ropi ( A. lwoffii ), ahsine Saturday bakteo on the Tiber jiniah (A. bereziniae ), ahsine Saturday bakteo to Mori T Caicos ( A. haemolyticus ), Sat ahsine bakteo Parque booth ( A. parvus ) and ahsine Saturday bakteo swikdeul Larry ( A. schindleri ) promptly presence and the species name of a specific and has very beneficial effects that can accurately identify. ;
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机译：本发明的用于检测引物，探针的ahsine Saturday bakteo种类以及ahsine Sat的使用它们涉及一种检测bakteo种类的方法。更具体地说，本发明具有第一对引物，其由SEQ ID NO：1和2的核苷酸序列组成;第二对引物由SEQ ID NO：1和2的核苷酸序列组成。 SEQ ID NO：3和第二对引物，其由4的碱基序列组成; SEQ ID NO：5，和第三对引物组成的核苷酸序列6;第四对引物，其由SEQ ID NO：7和8的核苷酸序列组成。 SEQ ID NO：9和10，由第五对引物组成的核苷酸序列;第六对引物，其由SEQ ID NO：11和12的核苷酸序列组成;第七对引物，其由SEQ ID NO：13和14的核苷酸序列组成; SEQ ID NO：15，以及由核苷酸序列16组成的第八对引物;由碱基序列组成的一对引物的SEQ ID NO：17和9，18。 SEQ ID NO：19以及选自由10至20个核苷酸序列组成的组的至少一个引物，该引物包含一个选定的引物对ahsine sat bakteo（不动杆菌）引物，可以特异性地检测ahsine种类并使用Sat引物本发明涉及一种检测batteo种类的方法。根据本发明的使用该引物检测Ahsine Saturday Bakteo的Ahsine Saturday Bakteo方法是根据传染病医院星期六的Ahsine Bakteo刀Core Seti Syracuse（不动杆菌 calcoaceticus ），ahsine Saturday bakteo Bauer Mani（ A. baumannii ），ahsine Saturday bakteo Petey（ A. pittii ），是周六的爱丽丝·科米耶（ A. nosocomialis ）。 ahsine Saturday bakteo伯爵目的地（ A ursingii）， ahsine Saturday bakteo ropi（ A. lwoffii ），在台伯吉尼雅（A. bereziniae ）上的ahsine星期六bakteo ， ahsine星期六bakteo到Mori T Caicos（ A. < / I> haemolyticus ），Sat ahsine bakteo Parque展位（ A. parvus ）和ahsine Saturday bakteo swikdeul Larry（ A. < / I> schindleri ）迅速存在，并且具有特定的物种名称，并且具有可以准确识别的非常有益的效果。 ;
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