首页> 外国专利> COMPOSITION FOR SCREENING TYPES OF ORYZA SATIVA sp. L.HAVING BAKANAE DISEASE-RESISTANT BK1 GENE WITH DNA MARKER AND METHOD OF SCREENING ORYZA SATIVA L. HAVING BAKANAE DISEASE-RESISTANT BK1 GENE USING DNA MARKER

COMPOSITION FOR SCREENING TYPES OF ORYZA SATIVA sp. L.HAVING BAKANAE DISEASE-RESISTANT BK1 GENE WITH DNA MARKER AND METHOD OF SCREENING ORYZA SATIVA L. HAVING BAKANAE DISEASE-RESISTANT BK1 GENE USING DNA MARKER

机译:筛选ORYZA SATIVA sp。类型的组合物。 L.具有DNA标记的Bakanae抗病BK1基因和筛选Oryza Sativa L.利用DNA标记的Bakanae抗病BK1基因

摘要

The present invention relates to a composition used for screening types of Oryza sativa sp. L._having resistance to bakanae disease including primers to detect at least one oligonucleotide or pieces thereof and, more specifically, a composition used for screening types of Oryza sativa sp. L. having resistance to bakanae disease including a pair of primers selected from a group made from 3 to 16 sequence numbers. In addition, the present invention relates to a method for screening types of Oryza sativa sp. L. having resistance to bakanae disease, comprising the steps of separating genomic DNA from samples of Oryza sativa sp. L._; making the separated genomic DNA as a template DNA and performing amplification reaction for the same with use of at least one pair of primers selected from a group made from 3 to 16 sequence numbers, amplifying target sequences; and detecting the amplified, and a kit used for screening Oryza sativa sp. L._where the method for screening types of Oryza sativa sp. L._having resistance to bakanae diseases uses markers related to the position of a bakanae disease-resistant gene and accordingly screens sensitivity and resistance to the bakanae disease in an efficient manner wherein the molecular marker is used for culturing Oryza sativa sp. L._with improved resistance to bakanae diseases via MAS.;COPYRIGHT KIPO 2015
机译:本发明涉及用于筛选稻的类型的组合物。具有对巴卡那病的抗性,包括用于检测至少一种寡核苷酸或其片段的引物,更具体而言,是用于筛选稻的类型的组合物。具有对巴卡那病的抗性的L.包括一对引物,所述引物选自3至16个序列号。另外,本发明涉及用于筛选稻的类型的方法。对巴卡那病具有抗性的L.,包括从稻(Oryza sativa sp。)样品中分离基因组DNA的步骤。 L._;以分离的基因组DNA为模板DNA,并使用选自3至16个序列号的组中的至少一对引物,对其进行扩增反应,扩增靶序列。并检测扩增的产物,以及用于筛选水稻的试剂盒。 L._其中用于筛选水稻的类型的方法。具有对巴卡那病的抗性的L.使用与巴卡那病抗性基因的位置有关的标记,并因此以有效的方式筛选对巴卡那病的敏感性和抗性,其中该分子标记用于培养稻。 L._通过MAS提高了对巴卡那病的抵抗力; COPYRIGHT KIPO 2015

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