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Method for mass producing TEV protease in plant and TEV protease produced by the same

机译:在植物中大量生产TEV蛋白酶的方法和由其生产的tev蛋白酶

摘要

The present invention provides a plant expression recombinant vector for mass producing tobacco etch virus (TEV) protease comprising genes coding TEV derived protease; a method for mass producing the TEV protease from plant comprising a step of overexpressing the genes coding TEV derived protease by transforming plant cells using the recombinant vector; a method for preparing a genetically modified organism which mass produces the TEV protease comprising a step of transforming plant cells using the recombinant vector; the genetically modified organism which mass produces the TEV protease prepared by the method, and seeds thereof; a detergent composition, a food composition, and a forage composition comprising the protease isolated from the genetically modified organism as an active ingredient; and a composition for mass producing the TEV protease from plant comprising the genes coding protease derived from the TEV consisting of base sequence described in sequence number 1.
机译:本发明提供了用于大规模生产烟草蚀刻病毒(TEV)蛋白酶的植物表达重组载体,其包含编码TEV衍生的蛋白酶的基因。一种从植物中大量生产TEV蛋白酶的方法,该方法包括通过使用重组载体转化植物细胞来过表达编码TEV衍生蛋白酶的基因的步骤。一种制备大量生产TEV蛋白酶的转基因生物的方法,该方法包括使用重组载体转化植物细胞的步骤。大量生产通过该方法制备的TEV蛋白酶的转基因生物及其种子;洗涤剂组合物,食品组合物和草料组合物,其包含从转基因生物分离的蛋白酶作为活性成分;以及从植物中大量生产TEV蛋白酶的组合物,其包含编码由TEV衍生的蛋白酶的基因,该基因由序列号1中所述的碱基序列组成。

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