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TECHNIQUE COMBINING PCR AND LOOP-MEDIATED ISOTHERMAL AMPLIFICATION FOR THE DETECTION OF NUCLEIC ACIDS
TECHNIQUE COMBINING PCR AND LOOP-MEDIATED ISOTHERMAL AMPLIFICATION FOR THE DETECTION OF NUCLEIC ACIDS
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机译：PCR和环介导的等温扩增相结合的核酸检测技术
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摘要
The present invention relates to a method and a kit of parts for detecting the presence or absence of one or more target nucleic acid sequences in a sample, the method comprising a sequence of steps for pre-amplifying the sample by means of a polymerase chain reaction, followed by a sequence of steps comprising an isothermal amplification of the pre-amplified sample, wherein the isothermal amplification comprises a pair of primers comprising a forward primer having a 3′ part that is substantially complementary to a first part of the target sequence, the presence or absence of which is to be detected, and a 5′ part that is substantially homolog to a second part of the target sequence, and a reverse primer comprising a 3′ part that is substantially homolog to a fourth part of the target sequence and a 5′ part that is substantially complementary to a third part of the target sequence.
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