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ARTIFICIAL CELL MEMBRANE COMPRISING SUPPORTED LIPID BILAYER IN WHICH METAL PARTICLE PROBE HAVING CONTROLLED MOBILITY IS COUPLED, AND METHOD FOR ANALYZING MOLECULAR INTERACTION BY USING SAME
ARTIFICIAL CELL MEMBRANE COMPRISING SUPPORTED LIPID BILAYER IN WHICH METAL PARTICLE PROBE HAVING CONTROLLED MOBILITY IS COUPLED, AND METHOD FOR ANALYZING MOLECULAR INTERACTION BY USING SAME
The present invention relates to: an artificial cell membrane comprising a substrate and a supported lipid bilayer (SLB) in which metal particles having decreased mobility are coupled onto the substrate; an analysis apparatus or a kit for ascertaining molecular interaction, comprising the artificial cell membrane, wherein one molecule is coupled to the surface of metal particles having decreased mobility coupled to the artificial cell membrane, and the other molecule is coupled to the surface of metal particles having wide mobility coupled to a lipid by a low binding value; a method for ascertaining molecular interaction by using the analysis apparatus; a kit for quantitative or qualitative analysis of a target material, containing the artificial cell membrane, by using a plasmon scattering measurement; and a multiple analysis kit capable of detecting a plurality of target materials by using a plurality of metal particles having different plasmon scattering wavelengths and/or mobility on an SLB. The artificial cell membrane containing an SLB in which metal particles are coupled, of the present invention, allows the mobility of metal particles to be controlled on a lipid by controlling the number of ligands coupled to metal particles. Therefore, target molecules, of which the interaction is to be analyzed, are respectively introduced onto two types of metal particles having different mobilities so as to be introduced onto the artificial cell membrane, and thus the movement of the metal particles is monitored by plasmon scattering, thereby enabling the analysis of the interaction between the target molecules. Multiple analyses of simultaneously detecting and quantifying a plurality of target materials is enabled by using the artificial cell membrane of the present invention and a plurality of particles having different plasmon scattering wavelengths or having different mobilities on a lipid.
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