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COMPOSITIONS AND METHODS FOR GALLS FL AND GALLS CT MEDIATED TRANSFORMATION OF PLANTS
COMPOSITIONS AND METHODS FOR GALLS FL AND GALLS CT MEDIATED TRANSFORMATION OF PLANTS
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机译：Galls FL和Galls CT介导的植物转化的组成和方法
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摘要
The present disclosure is directed to compositions and related methods that incorporate GALLS full-length (FL) or CT domain proteins to enhance efficiency of genetic manipulation of plants. In one aspect, the disclosure provides a modified Agrobacterium cell that comprises a first nucleic acid and a second nucleic acid that encodes a GALLS-FL protein. In another aspect, the disclosure provides a method of enhancing the single copy insertion of a first nucleic acid sequence into a plant cell genome. In another aspect, the disclosure provides a method of inducing plant susceptibility to Agrobacterium-mediated transformation, comprising providing GALLS- CT polypeptide in the cytosol of at least one cell of the plant. In another aspect, the disclosure provides a transgenic plant that comprises a heterologous nucleic acid sequence encoding GALLS-CT operably linked to a promoter sequence.
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