首页> 外国专利> APPLICATION OF ENZYME-QUANTUM DOTS HYBRID SYSTEM FOR THE DETERMINATION OF URIC ACID

APPLICATION OF ENZYME-QUANTUM DOTS HYBRID SYSTEM FOR THE DETERMINATION OF URIC ACID

机译:酶-量子点混合系统在尿酸测定中的应用

摘要

The present invention relates to a uricase/HRP-CdS QDs hydrid system for the determination of uric acid. The QDs was used as an electron donor, whereas uricase and HRP (horseradish peroxidase) were used as acceptors for the oxidation/reduction reactions involved in oxidizing uric acid to allaintoin and hydrogen peroxide. The hydrogen peroxide produced was able to quench the QDs fluorescence which was proportional to uric acid concentration. The hybrid system demonstrated maximum activity of uricase/HRP at ratio of 1/1 and pH 7.0, respectively. The linearity of the hybrid system towards uric acid was in the concentration range of 125-1000 μΜ with detection limit of 125 μΜ.
机译:本发明涉及一种用于测定尿酸的尿酸酶/ HRP-CdS QDs氢化物系统。量子点被用作电子供体,而尿酸酶和HRP(辣根过氧化物酶)被用作将尿酸氧化成尿囊素和过氧化氢的氧化/还原反应的受体。产生的过氧化氢能够猝灭与尿酸浓度成比例的QDs荧光。混合系统分别以1/1和pH 7.0的比例显示了尿酸酶/ HRP的最大活性。杂合系统对尿酸的线性在125-1000μM的浓度范围内,检测极限为125μM。

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