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METHOD FOR DETECTING TRANSFER OF MAGNAPORTHE GRISEA EFFECTOR PROTEIN IN RICE ROOT TISSUE

机译:水稻根组织中食蟹猕猴效应蛋白转移的方法

摘要

A method for detecting transfer of magnaporthe grisea effector protein in a rice root tissue. The method comprises the following steps: verifying a magnaporthe grisea pathogen associated molecular pattern MgNIP53; treating a rice root tip by using a fusion protein; treating rice by using an immunofluorescent staining method, and observing transfer of the fusion protein in a rice root tissue. According to the method, a magnaporthe grisea pathogen associated molecular pattern MgNIP53 is authenticated by using a series of methods, a rice root tip is treated by using a fusion protein, and finally, a rice root tip is treated by using an immunofluorescent method, so as to observe the transfer of the fusion protein in a rice root tissue. The method is simple, accurate and quick, thereby providing a strong reference for developing a biological agent for biologically preventing the rice blast effectively in the future.
机译:一种检测稻瘟病效应蛋白在水稻根组织中转移的方法。该方法包括以下步骤:验证稻瘟病菌病原体相关分子模式MgNIP53;通过使用融合蛋白处理水稻根尖;使用免疫荧光染色法处理水稻,并观察融合蛋白在水稻根组织中的转移。根据该方法,通过一系列方法对稻瘟病菌病原体相关分子模式MgNIP53进行鉴定,通过融合蛋白处理稻根尖,最后通过免疫荧光法处理稻根尖,因此以观察融合蛋白在水稻根组织中的转移。该方法简单,准确,快速,为今后开发有效地生物防治稻瘟病的生物制剂提供了有力参考。

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