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METHOD FOR DETECTING TRANSFER OF MAGNAPORTHE GRISEA EFFECTOR PROTEIN IN RICE ROOT TISSUE
METHOD FOR DETECTING TRANSFER OF MAGNAPORTHE GRISEA EFFECTOR PROTEIN IN RICE ROOT TISSUE
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机译:水稻根组织中食蟹猕猴效应蛋白转移的方法
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摘要
A method for detecting transfer of magnaporthe grisea effector protein in a rice root tissue. The method comprises the following steps: verifying a magnaporthe grisea pathogen associated molecular pattern MgNIP53; treating a rice root tip by using a fusion protein; treating rice by using an immunofluorescent staining method, and observing transfer of the fusion protein in a rice root tissue. According to the method, a magnaporthe grisea pathogen associated molecular pattern MgNIP53 is authenticated by using a series of methods, a rice root tip is treated by using a fusion protein, and finally, a rice root tip is treated by using an immunofluorescent method, so as to observe the transfer of the fusion protein in a rice root tissue. The method is simple, accurate and quick, thereby providing a strong reference for developing a biological agent for biologically preventing the rice blast effectively in the future.
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