首页> 外国专利> KIT AND METHOD FOR THE DETECTION, IDENTIFICATION AND DIFFERENTIAL DIAGNOSIS OF THE DENGUE VIRUS, WEST NILE VIRUS, RICKETTSIA SPP. AND LEPTOSPIRA SPP. BY PCR IN REAL TIME.

KIT AND METHOD FOR THE DETECTION, IDENTIFICATION AND DIFFERENTIAL DIAGNOSIS OF THE DENGUE VIRUS, WEST NILE VIRUS, RICKETTSIA SPP. AND LEPTOSPIRA SPP. BY PCR IN REAL TIME.

机译:登革热病毒,西尼罗河病毒,立克次体SPP的检测,鉴定和鉴别诊断的套件和方法。和LEPTOSPIRA SPP。通过PCR实时进行。

摘要

The present invention consists in mixtures of oligonucleotides acting as reaction primers and lyophilized or not buffers, useful for the detection and specific identification of infectious agents, specially 4 emerging pathogens able to produce disease with febrile symptoms, said detection or identification being performed by the molecular diagnosis, which may be carried out from the extraction of nucleic acids of clinical samples of patients. Said agents to be detected include but are not limited to: I) Dengue Virus, II) West Nile Virus, III) Ricktettsia spp., IV) Leptospira spp. In addition, the invention includes positive cloned controls performed to the sample.
机译:本发明包括用作反应引物和冻干或不冻干的寡核苷酸的混合物,可用于检测和特异性鉴定传染病,特别是能够产生发热症状的疾病的4种新兴病原体,所述检测或鉴定由分子进行诊断可以从患者临床样品的核酸提取中进行。待检测的所述试剂包括但不限于:I)登革热病毒,II)西尼罗河病毒,III)立克次氏体,IV)钩端螺旋体。另外,本发明包括对样品进行的阳性克隆对照。

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