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首页> 外国专利> The Method includes cultivation and Expansion of cells in ascitic Fluid Origin of peritoneal, pleural, peritoneal lavage washings or systemic allows to determine the best treatment for Patients with cancer.

The Method includes cultivation and Expansion of cells in ascitic Fluid Origin of peritoneal, pleural, peritoneal lavage washings or systemic allows to determine the best treatment for Patients with cancer.

机译：该方法包括在腹水，胸膜，腹膜灌洗液或全身性腹水中培养和扩增腹水中的细胞，以确定对癌症患者的最佳治疗方法。

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The invention relates to a diagnosis method which is used outside the human or animal body and which can be used to identify and/or predict the best cancer treatment for a patient, said method comprising: i) depositing a sample of ascites fluid from a patient in a centrifuge and centrifuging the sample in order to obtain a first phase containing cells and a second phase corresponding to cell-free fluid; ii) analysing the second phase in order to check for the presence of at least 10 platelets per ml; iii) expanding the cells present in the first phase in a culture medium until 80% confluence is obtained; iv) selecting the cells expanded in step (iii), performing a cell dissociation step with a proteolytic enzyme or using a calcium chelating agent and subsequently, from the dissociated cells, determining molecular and/or cellular markers, in order to confirm the presence of a mixed cell population; and v) determining the best treatment for patients by: a) adding a dose of a chemotherapy drug to the cells from step (iv); b) using a viability assay to measure the degree of toxicity of each drug in the cells; c) repeating steps (a) and (b) with different chemotherapy drugs; and d) analysing the results obtained, whereby a response below 30% toxicity reflects resistance to treatment, a response above 30% toxicity corresponds to intermediate sensitivity to treatment, and a response above 50% toxicity reflects a high probability of response to treatment.
机译：本发明涉及一种在人或动物体外使用的诊断方法，该方法可用于识别和/或预测对患者的最佳癌症治疗，所述方法包括：i）沉积来自患者的腹水样品在离心和离心样品中以获得包含细胞的第一相和对应于无细胞流体的第二相; ii）分析第二阶段，以检查每毫升至少存在10个血小板; iii）在培养基中扩增第一相中存在的细胞，直到获得80％的融合; iv）选择在步骤（iii）中扩增的细胞，用蛋白水解酶或使用钙螯合剂进行细胞解离步骤，然后从解离的细胞中确定分子和/或细胞标记，以确认是否存在混合细胞群; v）通过以下方式确定对患者最好的治疗方法：a）向步骤（iv）的细胞中添加一定剂量的化学治疗药物; b）使用生存力测定法测量细胞中每种药物的毒性程度; c）用不同的化疗药物重复步骤（a）和（b）; d）分析所得结果，其中毒性低于30％的反应反映了对治疗的抵抗力，毒性高于30％的反应反映了对治疗的中等敏感性，毒性高于50％的反应反映了对治疗的高可能性。

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