首页>
外国专利>
Production method of iduronate-2-sulfatase (I2S), which includes cultivating Mammalian Cells that coexpressed recombinant Enzyme i2S and generating a formilglicina (FGE); preparation of Protein i2S; Pharmaceutical Composition and use for treating Hunter Syndrome.
Production method of iduronate-2-sulfatase (I2S), which includes cultivating Mammalian Cells that coexpressed recombinant Enzyme i2S and generating a formilglicina (FGE); preparation of Protein i2S; Pharmaceutical Composition and use for treating Hunter Syndrome.
The present invention provides, among other things, methods and compositions for large-scale production of recombinant I2S protein using suspension culture of mammalian cells in serum-free medium. In particular, the present invention uses mammalian cells co-express a recombinant I2S protein and a formylglycine generating enzyme (FGE).
展开▼
