首页> 外国专利> Construction, method of production of plant cells or plants transplastu00f4micas free of selection marker, method of production of plant cells or plants legumes transplastu00f4micas free of selection marker, cell transplastu00f4mica vegetable legume plant, cell CA Ansplastu00f4mica, legume seed of legume plant transplastu00f4mica, transplastu00f4mica,The seed of legume and oil or food

Construction, method of production of plant cells or plants transplastu00f4micas free of selection marker, method of production of plant cells or plants legumes transplastu00f4micas free of selection marker, cell transplastu00f4mica vegetable legume plant, cell CA Ansplastu00f4mica, legume seed of legume plant transplastu00f4mica, transplastu00f4mica,The seed of legume and oil or food

机译：不含选择标记的植物细胞或植物的构建，生产方法，无选择标记的植物细胞或植物豆科植物，不含选择标记的植物细胞或植物的生产方法，细胞transplast u00f4mica，植物豆科植物，细胞CA Ansplast u00f4mica，豆类豆科植物的种子转化塑料，转化豆类，油或食物的种子

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They also provide a cell of a Legume Plant and transplastu00f3mica transplastu00f3mica modified with the installation described above and an oil or food derived from the transplastu00f3mica processing plant or seed.Claim 1: Assembly comprising, in the direction of transcription, at least (i) a Chimeric Gene encodes a selection Marker that confers resistance to a selection Agent, and (ii) a Chimeric Gene variation, or Chimeric Gene encodes a Protein product, or g In Chimeric encodes a negative Marker.Both inserted between the terminal sequence of the 5 'and 3' terminal sequence, respectively, of a Chimeric Gene of interest, in which these two sequences are superimposed on a fragment has a length sufficient to allow recombinationAnd in which the Gene of interest is not present as an entity of full length when the Chimeric gene (ii) encodes a negative Marker.Claim 6: a method for obtaining plant cells or plants transplastu00f3micas free of selection Marker which comprises at least the following Stages: a) at least one Plant Cell transformed with a Vector suitable for plastid Transformation, which includes (i) a Chimeric Gene encodes a selection Marker that confers resistance to an agentSelection and (ii) a Chimeric Gene variation, or Chimeric Gene encodes a Protein product, both inserted between the terminal sequence of the 5 'and 3' terminal sequence, respectively, of a Chimeric Gene of interestIn These two sequences are superimposed on a fragment has a length sufficient to allow recombination; (b) cultivate the cell or cells that comprise the plastid transformed into a first half that includes the selection agent; (c) cultivate the cell or cu00e9lu In the second half, not Understanding the selection agent; (d) to select the cell or cells of theWhen a plant is used (ii) Chimeric Gene encodes a Protein product, or select the cell or cells of the plant or plants not coloured (ii) when using a Chimeric Gene Variation.

机译：它们还提供了一种豆科植物细胞和经上述装置改造的转质体细胞，以及衍生自该转质体加工厂或种子的油或食品。权利要求1：装配体，在转录方向上包括：至少（i）嵌合基因编码赋予选择剂抗性的选择标记，和（ii）嵌合基因变异或嵌合基因编码蛋白质产物，或g In嵌合编码负标记。目的嵌合基因的5'和3'末端序列的末端序列，其中这两个序列重叠在一个片段上，其长度足以允许重组，并且其中目的基因不作为实体存在权利要求6：一种获得不含选择标记的植物细胞或植物质体的方法，该方法至少包括：以下阶段：a）至少一种用适合质体转化的载体转化的植物细胞，其中包括（i）嵌合基因编码赋予对试剂选择抗性的选择标记，以及（ii）嵌合基因变异，或嵌合基因编码蛋白产物，分别插入感兴趣的嵌合基因的5'和3'末端序列的末端序列之间。这两个序列重叠在片段上，其长度足以允许重组; （b）培养包含被转化为包含选择剂的上半部分的质体的一个或多个细胞; （c）在下半年培养细胞或不了解选择剂; （d）选择使用植物时的一个或多个细胞（ii）嵌合基因编码蛋白质产物，或使用嵌合基因变异时选择未着色的一个或多个植物的细胞或细胞。

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公开/公告号BRPI0919366A2

专利类型
公开/公告日2015-08-18

原文格式PDF
申请/专利权人 BAYER CROPSCIENCE AG;
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申请/专利号BR2009PI19366
发明设计人 MANUEL DUBALD;ANNE ROLLAND;BERNARD PELISSIER;CHRISTELLE LESTRADE;
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申请日2009-12-31
分类号C12N15/82;A01H5;
国家 BR
入库时间 2022-08-21 15:15:55

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